PIKE Antibody

Product Code: PSI-3321

Supplier: ProSci

Code	Size	Price

PSI-3321-0.02mg

0.02mg

£150.00

Quantity:

PSI-3321-0.1mg

0.1mg

£449.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit

Antibody Isotype: IgG

Antibody Clonality: Polyclonal

Regulatory Status: RUO

Applications:

Enzyme-Linked Immunosorbent Assay (ELISA)
Immunohistochemistry (IHC)
Western Blot (WB)

Images

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Western blot analysis of PIKE in mouse brain cell lysates with PIKE antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.

2 / 2

Immunohistochemistry of PIKE in mouse brain tissue with PIKE antibody at 10 μg/mL.

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Documents

Further Information

Additional Names:

PIKE Antibody: PIKE, GGAP2, CENTG1, KIAA0167, Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2, Centaurin-gamma-1, AGAP-2

Application Note:

PIKE antibody can be used for detection of PIKE by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.

Antibody validated: Western Blot in mouse samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.

Background:

PIKE Antibody: Phosphoinositide 3 kinase enhancer (PIKE) is a recently identified nuclear GTPase that interacts with nuclear phosphoinositide 3-kinase (PI3 kinase) to stimulate its lipid kinase activity. PIKE exists in multiple isoforms; a shorter C-terminal isoform (PIKE-A) has also been identified as centaurin gamma 1. The longest isoform (PIKE-L) has been shown to bind to the adaptor protein Homer and thereby link to metabotropic glutamate receptors, leading to activation of PI3 kinase activity and prevention of neuronal apoptosis. Overexpression of PIKE-A enhances Akt activity and promotes cancer cell invasion, whereas decreased expression of PIKE-A via dominant negative expression of PIKE-A or PIKE-A knockdown inhibits these processes. In many human cancers, expression of PIKE-A is enhanced, leading to increased Akt activity and preventing apoptosis.

Background References:

Ye K, Hurt KJ, Wu FY, et al. Pike. A nuclear gtpase that enhances PI3kinase activity and is regulated by protein 4.1N. Cell 2000; 103:919-30.
Jackson TR, Kearns BG, and Thiebert AB. Cytohesins and centaurins: mediators of PI3-kinase-regulated Arf signaling. Trends Biochem. Sci. 2000; 25:489-95.
Rong R, Ahn JY, Huang H, et al. PI3 kinase enhancer-Homer complex couples mGluRI to PI3 kinase, preventing neuronal apoptosis. Nat. Neurosci. 2003; 6:1153-61.
Ahn J-Y, Rong R, Kroll TG, et al. PIKE (Phosphotidylinositol 3-kinase enhancer)-A GTPase stimulates Akt activity and mediates cellular invasion. J. Biol. Chem. 2004; 279:16441-51.

Buffer:

PIKE Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration:

1 mg/mL

Conjugate:

Unconjugated

DISCLAIMER:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Homology:

Predicted species reactivity based on immunogen sequence: Rat: (86%)

Immunogen:

PIKE antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human PIKE.

The immunogen is located within the last 50 amino acids of PIKE.

NCBI Gene ID #:

116986

NCBI Official Name:

ArfGAP with GTPase domain, ankyrin repeat and PH domain 2

NCBI Official Symbol:

AGAP2

NCBI Organism:

Homo sapiens

Physical State:

Liquid

Protein Accession #:

AAM97540

Protein GI Number:

25989575

Purification:

PIKE Antibody is Ion exchange chromatography purified.

Research Area:

Apoptosis

SPECIFICITY:

Anti-PIKE will detect both PIKE-L and PIKE-A isoforms.

Swissprot #:

Q99490

User NOte:

Optimal dilutions for each application to be determined by the researcher.

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