cIAP Antibody

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ProSci
Product Code: PSI-3325
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3325-0.02mg0.02mg£150.00
Quantity:
PSI-3325-0.1mg0.1mg£449.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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<strong>Figure 1 Western Blot Validation in Human Lung Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: cIAP 3325 (A: 1 μg/mL, B: 2 μg/mL and C: 4 μg/mL ), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Human, Mouse and Rat Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: cIAP 3325 (2 μg/mL), cIAP, competitor antibody, (1 μg/mL) and beta-actin 3779 (1.5 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 3 Western Blot Validation in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: cIAP 3325 (2 μg/mL ), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 4 Western Blot Validation in Human and Rat Tissue Lysates</strong><br> Loading: 15 μg of lysates per lane. Antibodies: cIAP 3325 (1 μg/mL ), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 5 Immunohistochemistry Validation of cIAP in Human Lung Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-cIAP antibody (3325) at 10 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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<strong>Figure 6 Immunofluorescence Validation of cIAP in Human Lung Tissue</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human lung tissue abeling cIAP with 3325 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).
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<strong>Figure 6 Immunofluorescence Validation of cIAP in Human Lung Tissue</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human lung tissue abeling cIAP with 3325 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).
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<strong>Figure 1 Western Blot Validation in Human Lung Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: cIAP 3325 (A: 1 μg/mL, B: 2 μg/mL and C: 4 μg/mL ), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Human, Mouse and Rat Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: cIAP 3325 (2 μg/mL), cIAP, competitor antibody, (1 μg/mL) and beta-actin 3779 (1.5 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: cIAP 3325 (2 μg/mL ), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Western Blot Validation in Human and Rat Tissue Lysates</strong><br> Loading: 15 μg of lysates per lane. Antibodies: cIAP 3325 (1 μg/mL ), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 5 Immunohistochemistry Validation of cIAP in Human Lung Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-cIAP antibody (3325) at 10 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 6 Immunofluorescence Validation of cIAP in Human Lung Tissue</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human lung tissue abeling cIAP with 3325 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).
<strong>Figure 6 Immunofluorescence Validation of cIAP in Human Lung Tissue</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human lung tissue abeling cIAP with 3325 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).

Documents

Further Information

Additional Names:
cIAP Antibody: API1, MIHB, HIAP2, RNF48, cIAP1, Hiap-2, c-IAP1, API1, IAP2, Baculoviral IAP repeat-containing protein 2, C-IAP1, IAP-2
Application Note:
WB: 1-4 μg/mL; IHC: 10 μg/mL; IF: 20 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
cIAP Antibody: Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and can be prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. The two isoforms of c-IAP (c-IAP1 and c-IAP2) are structurally related to XIAP, containing 3 baculoviral IAP repeat (BIR) motifs that are essential and sufficient for the binding and inhibition of caspases-3, -7. The c-IAPs can associate with the death receptor TNF-R2, and mediate the ubiquitinization of TRAF2 following the binding of TNF-α by its receptor. Omi, a negative regulator of c-IAP, inhibits its activity by catalytically cleaving c-IAP. Another negative regulator, Smac/DIABLO, acts by enhancing the auto-ubiquitization activity of c-IAP.
Background References:
  • Schimmer. Cancer Res. 2004; 64:7183-90.
  • Rothe et al. Cell 1995; 83:1243-52.
  • Deveraux et al. EMBO J. 1998; 17:2215-23.
  • Li et al. Nature 2002; 416:345-7.
Buffer:
cIAP Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (93%), Chicken: (92%)
Immunogen:
Anti-cIAP antibody (3325) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human cIAP. c-IAP antibody detects both c-IAP1 and c-IAP2.

The immunogen is located within amino acids 540-590 of cIAP.
ISOFORMS:
Human cIAP has 2 isoforms, including isoform 1 (618aa, 70kD) and isoform 2 (569aa, 64kD). Mouse cIAP has one isoform (612aa, 70kD) and Rat cIAP also has one isoform (589aa, 67kD). 3325 can detect human, mouse and rat.
NCBI Gene ID #:
329
NCBI Official Name:
baculoviral IAP repeat-containing 2
NCBI Official Symbol:
BIRC2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 70kD

Observed: 70kD
Protein Accession #:
NP_001157
Protein GI Number:
4502141
Purification:
cIAP Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis,Cancer
Swissprot #:
Q13490
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:

Independent Antibody Validation in Cell lines (Figure 2) shows similar cIAP expression profile in human, mouse and rat cell lines detected by two independent anti-cIAP antibodies that recognize different epitopes, 3325 against C-terminus domain and competitor antibody against recombinant protein.  cIAP proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. 

References

  1. Nuutinen et al. The effect of microenvironmental CD40 signals on TRAIL- and drug-induced apoptosis in follicular lymphoma cells. Scand J Immunol. 2009;70(6):565-73.PMID: 19906199
  2. Hahm and Singh. Withaferin A-induced apoptosis in human breast cancer cells is associated with suppression of inhibitor of apoptosis family protein expression. Cancer Lett. 2013;334(1):101-8. PMID: 22935676

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