SIGIRR Antibody

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ProSci
Product Code: PSI-3367
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3367-0.02mg0.02mg£150.00
Quantity:
PSI-3367-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of SIGIRR expression in (A) A549 and (B) MCF7 cell lysate with SIGIRR antibody at ?1 μg/ml.
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Immunocytochemistry of SIGIRR in A549 cells with SIGIRR antibody at 10 μg/mL.
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Western blot analysis of SIGIRR expression in (A) A549 and (B) MCF7 cell lysate with SIGIRR antibody at ?1 μg/ml.
Immunocytochemistry of SIGIRR in A549 cells with SIGIRR antibody at 10 μg/mL.

Documents

Further Information

Additional Names:
SIGIRR Antibody: TIR8, Single Ig IL-1-related receptor, Single Ig IL-1R-related molecule, TIR8
Application Note:
SIGIRR antibody can be used for the detection of SIGIRR by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.

Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Background:
SIGIRR Antibody: SIGIRR is a member of the Toll-like receptor-interleukin 1 receptor superfamily. Members of this family are defined by the presence of an intracellular Toll-IL-1R (TIR) domain. The Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. SIGIRR was originally identified through database analysis and was shown to have only one Ig domain as opposed to the normal three Ig folds seen in the TIR family. Similar to ST2, another TIR family member, it has been shown to negatively regulate IL-1 receptor and Toll-like receptor signaling. However, SIGIRR inhibits TLR-IL-1R signaling by dimerizing with TLR4, TLR5, TLR9, and IL-1R. It also associates with the down-stream TLR signaling proteins IRAK and TRAF6 in an IL-1-dependent fashion.
Background References:
  • Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76.
  • Janeway CA Jr. and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002; 20:197-216.
  • Thomassen E, Renshaw BR, and Sims JE. Identification and characterization of SIGIRR, a molecule representing a novel subtype of the IL-1R superfamily. Cytokine 1999; 11:389-99.
  • Wald D, Qin J, Zhao Z, et al. SIGIRR, a negative regulator of Toll-like receptor-interleukin 1 receptor signaling. Nat. Immunol. 2003; 4:920-7.
Buffer:
SIGIRR Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (100%)
Immunogen:
SIGIRR antibody was raised against a synthetic peptide corresponding to 15 amino acids at the C-terminus of human SIGIRR.

The immunogen is located within the last 50 amino acids of SIGIRR.
NCBI Gene ID #:
59307
NCBI Official Name:
single immunoglobulin and toll-interleukin 1 receptor (TIR) domain
NCBI Official Symbol:
SIGIRR
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 45 kDa

Observed: 45 kDa?
Protein Accession #:
CAG33619
Protein GI Number:
48146793
Purification:
SIGIRR Antibody is affinity chromatography purified via peptide column.
Research Area:
Innate Immunity
Swissprot #:
Q6IA17
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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