UEV1A Antibody

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ProSci
Product Code: PSI-3375
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3375-0.02mg0.02mg£150.00
Quantity:
PSI-3375-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)

Images

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Western blot analysis of UEV1A in Jurkat cell lysates with UEV1A antibody at (A) 1, (B) 2, and (C) 4 μg/mL.
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Western blot analysis of UEV1A in Jurkat cell lysates with UEV1A antibody at (A) 1, (B) 2, and (C) 4 μg/mL.

Documents

Further Information

Additional Names:
UEV1A Antibody: CIR1, UEV1, CROC1, UBE2V, UEV-1, UEV1A, CROC-1, P/OKcl.19, Ubiquitin-conjugating enzyme E2 variant 1
Application Note:
UEV1A antibody can be used for detection of UEV1A by Western blot at 1 to 4 μg/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Background:
UEV1A Antibody: Ubiquitin-conjugating enzyme E2 (UEV1) was initially discovered as a protein similar in sequence and structure to the E2 ubiquitin-conjugating enzymes but lacking their enzymatic activity. There are at least two variants and multiple isoforms of UEV1. In particular, UEV1A (Ubiquitin-conjugating enzyme E2 variant 1 isoform A) has recently been shown to be an important component of the Toll-like receptor and IL-1R signaling pathway. Signals from these pathways are relayed by a number of downstream molecules such as MyD88 and tumor necrosis factor receptor associated factor (TRAF6), ultimately activating various kinases and transcription factors. UEV1A is part of a dimeric ubiquitin-conjugating enzyme complex also containing Ubc13 (ubiquitin-conjugating enzyme 13) that together with TRAF6 activates TAK1, a member of the mitogen-activated protein kinase kinase kinase family. The Ubc13-UEV1A complex also mediates the Lys-63 ubiquitination of TRAF-6, and this ubiquitination is essential for TAK1 activation.
Background References:
  • Sancho E, Vila MR, Sanchez-Pulido L, et al. Role of UEV-1, an inactive variant of the E2 ubiquitin-conjugating enzymes, in in vitro differentiation and cell cycle behavior of HT-29-M6 intestinal mucosecretory cells. Mol. Cell Biol. 1998; 18:576-89.
  • Akira S and Takeda K. Toll-like receptor Signalling. Nat. Rev. Immunol. 2004; 4:499-511.
  • Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77.
  • Deng L, Wang C, Spencer E, et al. Activation of the IκB kinase complex by TRAF6 requires a dimeric ubiquitin-conjugating enzyme complex and a unique polyubiquitin chain. Cell 2000; 103:351-61.
Buffer:
UEV1A Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Chicken: (100%)
Immunogen:
UEV1A antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human UEV1A.

The immunogen is located within the last 50 amino acids of UEV1A.
NCBI Gene ID #:
7335
NCBI Official Name:
ubiquitin-conjugating enzyme E2 variant 1
NCBI Official Symbol:
UBE2V1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_954595
Protein GI Number:
40806167
Purification:
UEV1A Antibody is Ion exchange chromatography purified.
Research Area:
Innate Immunity
SPECIFICITY:
Anti-UEV1A may also recognize other isoforms of UEV1A.
Swissprot #:
Q13404
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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