TAK1 Antibody

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ProSci
Product Code: PSI-3385
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3385-0.02mg0.02mg£150.00
Quantity:
PSI-3385-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)

Images

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Western blot analysis of TAK1 in Rat thymus cell lysate with TAK1 antibody at (A) 1, (B) 2, and (C) 4 μg/mL.
2 / 2
Immunofluorescence of TAK1 in Rat Thymus cells with TAK1 antibody at 10 μg/mL.
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Western blot analysis of TAK1 in Rat thymus cell lysate with TAK1 antibody at (A) 1, (B) 2, and (C) 4 μg/mL.
Immunofluorescence of TAK1 in Rat Thymus cells with TAK1 antibody at 10 μg/mL.

Documents

Further Information

Additional Names:
TAK1 Antibody: TAK1, MEKK7, TGF1a, TAK1, Mitogen-activated protein kinase kinase kinase 7, Transforming growth factor-beta-activated kinase 1, TGF-beta-activated kinase 1
Application Note:
TAK1 antibody can be used for the detection of TAK1 by Western blot at 1 to 4 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Background:
TAK1 Antibody: TAK1 (TGF-beta activated protein kinase 1) is a widely expressed enzyme originally identified as a mitogen-activated protein kinase kinase kinase (MAP3K7). It has since been shown to mediate various intracellular signaling pathways, such as those induced by TGF-beta and members of the Toll-IL-1R (TIR) superfamily, thus acting as an intermediate in both proliferative and innate and adaptive immune responses. TAK1 is normally present in cells in a complex with TAK1-binding protein 1 (TAB1) and either TAB2 or TAB3. Association with TAB1 triggers TAK1 kinase activity by inducing TAK1 autophosphorylation, while TAB2 and possibly TAB3 may contribute to SAPK2a/p38a-mediated feedback inhibition of TAK1 activity.
Background References:
  • Yamaguchi K, Shirakabe K, Shibuya H, et al. Identification of a member of the MAPKKK family as a potential mediator of TGF-β signal transduction. Science 1995; 270:2008-11.
  • Irie T, Muta T, and Takeshige K. TAK1 mediates an activation signal from toll-like receptor(s) to nuclear factor-κB in lipopolysaccharide-stimulated macrophages. FEBS Lett. 2000; 467:160-4.
  • Akira S and Takeda K. Toll-like receptor Signalling. Nat. Rev. Immunol. 2004; 4:499-511.
  • Jiang Z, Ninomiya-Tsuji J, Qian Y, et al. Interleukin-1 (IL-1) receptor-associated kinase-dependent IL-1-induced signaling complexes phosphorylate TAK1 and TAB2 at the plasma membrane and activate TAK1 in the cytosol. Mol. Cell Biol. 2002; 22:7158-67.
Buffer:
TAK1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
TAK1 antibody was raised against a synthetic peptide corresponding to 14 amino acids near the amino-terminus of human TAK1 isoform a.

The immunogen is located within amino acids 40 - 90 of TAK1.
NCBI Gene ID #:
6885
NCBI Official Name:
mitogen-activated protein kinase kinase kinase 7
NCBI Official Symbol:
MAP3K7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
BAA25025
Protein GI Number:
2924624
Purification:
TAK1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction,Cancer
Swissprot #:
O43318
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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