BTK Antibody

Contact us
ProSci
Product Code: PSI-3395
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3395-0.02mg0.02mg£150.00
Quantity:
PSI-3395-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

1 / 5
Western blot analysis of BTK in U937 cell lysate with BTK antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
2 / 5
Immunohistochemistry of BTK in mouse brain tissue with BTK antibody at 5 μg/mL.
3 / 5
Immunofluorescence of BTK in Daudi cells with BTK antibody at 10 μg/mL.
4 / 5
Immunocytochemistry of BTK in Daudi cells with BTK antibody at 10 μg/mL.
5 / 5
Immunofluorescence of BTK in mouse brain tissue with BTK antibody at 20 μg/ml.<br><br>Red: BTK Antibody (3395) <br> Blue: DAPI staining
❮❯

Western blot analysis of BTK in U937 cell lysate with BTK antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
Immunohistochemistry of BTK in mouse brain tissue with BTK antibody at 5 μg/mL.
Immunofluorescence of BTK in Daudi cells with BTK antibody at 10 μg/mL.
Immunocytochemistry of BTK in Daudi cells with BTK antibody at 10 μg/mL.
Immunofluorescence of BTK in mouse brain tissue with BTK antibody at 20 μg/ml.<br><br>Red: BTK Antibody (3395) <br> Blue: DAPI staining

Documents

Further Information

Additional Names:
BTK Antibody: AT, ATK, BPK, XLA, IMD1, AGMX1, PSCTK1, Tyrosine-protein kinase BTK, Agammaglobulinemia tyrosine kinase
Application Note:
BTK antibody can be used for the detection of BTK by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 10 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
Background:
BTK Antibody: Bruton's tyrosine kinase (BTK) was initially identified as a member of the src family for protein-tyrosine kinases that was involved in X-linked agamma-globulinaemia, and has since been shown to involved in a number of signaling pathways in hemapoietic lineage. It has recently been shown to interact with members of the toll-like receptor (TLR) family such as TLR4, 6, 8, and 9. The TLRs are critical molecules in both the innate and adaptive immunity and can recognize diverse microbial pathogens. BTK has also been shown to interact with key proteins involved in TLR4 signal transduction such as MyD88, TIRAP, and IRAK, but not TRAF-6, suggesting that BTK is involved in lipopolysaccharide signal transduction.
Background References:
  • Vetrie D, Vorechovsky I, Sideras P, et al. The gene involved in X-linked aγglobulinaemia is a member of the src family of tyrosine kinases. Nature 1993; 361:226-33.
  • Kawakami Y, Kitaura J, Hata D, et al. Functions of Bruton's tyrosine kinase in mast and B cells. J. Leukoc. Biol. 1999; 65(3):286-90.
  • Jefferies CA, Doyla S, Brunner C, et al. Bruton's tyrosine kinase is a Toll/Interleukin-1 receptor domain-binding protein that participates in nuclear factor kB activation by Toll-like receptor 4. J. Biol. Chem. 2003; 278:26258-26264.
  • Akira S and Takeda K. Toll-like receptor Signalling. Nat. Rev. Immunol. 2004; 4:499-511.
Buffer:
BTK Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (100%)
Immunogen:
BTK antibody was raised against a synthetic peptide corresponding to 16 amino acids near the amino-terminus of human BTK.

The immunogen is located within amino acids 20 - 70 of BTK.
NCBI Gene ID #:
695
NCBI Official Name:
Bruton agammaglobulinemia tyrosine kinase
NCBI Official Symbol:
BTK
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 76 kDa

Observed: 79 kDa
Protein Accession #:
Q06187
Protein GI Number:
547759
Purification:
BTK Antibody is affinity chromatography purified via peptide column.
Research Area:
Innate Immunity
SPECIFICITY:
Multiple isoforms of BTK are known to exist.
Swissprot #:
Q06187
User NOte:
Optimal dilutions for each application to be determined by the researcher.

Related Products

Product NameProduct CodeSupplier 
BTK PeptidePSI-3395PProSci Summary Details