IRF8 Antibody

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ProSci
Product Code: PSI-3401
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3401-0.02mg0.02mg£150.00
Quantity:
PSI-3401-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)

Images

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Western blot analysis of IRF8 in human thymus tissue lysate with IRF8 antibody at 1μg/mL in (A) the presence and (B) absence of blocking peptide.
2 / 2
Immunofluorescence of IRF8 in K562 cells with IRF8 antibody at 10 μg/mL.
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Western blot analysis of IRF8 in human thymus tissue lysate with IRF8 antibody at 1μg/mL in (A) the presence and (B) absence of blocking peptide.
Immunofluorescence of IRF8 in K562 cells with IRF8 antibody at 10 μg/mL.

Documents

Further Information

Additional Names:
IRF8 Antibody: Interferon Regulatory Factor, Interferon Consensus Sequence Binding Protein, H-ICSBP, ICSBP1, ICSBP, IRF-8, Interferon Consensus Sequence-Binding Protein, IMD32A, IMD32B
Application Note:
IRF8 antibody can be used for detection of IRF8 by Western blot at 1 μg/mL. For immunofluorescence start at 10 μg/mL.

Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
IRF8 Antibody: Interferons (IFN)s are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms. During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-alpha/beta genes. Unlike IRF3, IRF8 appears to act as a negative regulator of IFN-induced genes in most cases, but IRF8 mediates activation of NF-κB by the toll-like receptor 9 (TLR9) after stimulation by unmethylated CpG DNA in dendritic cells. Finally, it has been shown that IRF8 decreases bcl-2 expression and thus may play a role in chronic myelogenous leukemia.
Background References:
  • Malmgaard L. Induction and regulation of IFNs during viral infections. J. Interferon & Cyto. Res. 2004; 24:439-54
  • Weisz A, Marx P, Sharf R, et al. The human interferon consensus sequence binding protein (H-ICSBP) is a negative regulator of enhancer elements common to interferon inducible genes. J. Biol. Chem. 1992; 267:25589-96.
  • Nelson N, Marks MS, Driggers PH, et al. Interferon consensus sequence-binding protein, a member of the interferon regulatory factor family, suppresses interferon-induced gene transcription. Mol. Cell. Biol. 1993; 13:588-99.
  • Tsujimura H, Tamura T, Kong HJ, et al. Toll-like receptor 9 signaling activates NF-κB through IFN regulatory factor-8/IFN consensus sequence binding protein in dendritic cells. J. Immunol. 2004; 172:6820-7.
Buffer:
IRF8 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
IRF8 antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human IRF8.

The immunogen is located within the last 50 amino acids of IRF8.
NCBI Gene ID #:
3394
NCBI Official Name:
interferon regulatory factor 8
NCBI Official Symbol:
IRF8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
Q02556
Purification:
IRF8 Antibody is affinity chromatography purified via peptide column.
Research Area:
Chemokines & Cytokines
Swissprot #:
Q02556
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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