Anthrax Edema Factor Antibody
| Code | Size | Price |
|---|
| PSI-3419-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-3419-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Documents
Further Information
Additional Names:
Anthrax Edema Factor Antibody: Calmodulin-sensitive adenylate cyclase, ATP pyrophosphate-lyase, EF
Application Note:
Anthrax Edema Factor antibody can be used for the detection of Anthrax PA protein in ELISA. It will detect 10 ng of free peptide at 1 μg/mL.
Background:
Anthrax Edema Factor Antibody: Anthrax infection is initiated by the inhalation, ingestion, or cutaneous contact with Bacillus anthracis endospores. B. anthracis produces three polypeptides that comprise the anthrax toxin: protective antigen (PA), lethal factor (LF), and edema factor (EF). PA binds to two related proteins on the cell surface; these are termed tumor epithelial marker 8 (TEM8)/anthrax toxin receptor (ATR) and capillary morphogenesis protein 2 (CMG2), although it is still unclear which is physiologically relevant. Following PA binding to its receptor, PA is cleaved into two fragments by a furin-like protease. The bound fragment binds both LF and EF; the resulting complex is then endocytosed which allows the translocation of LF and EF into the cytoplasm. EF is a calmodulin and Ca++-dependent adenylate cyclase responsible for the edema seen in the disease. It is thought to benefit the B. anthracis bacteria by inhibiting cells of the host immune system.
Background References:
- Schwartz MN. Recognition and management of anthrax - an update. New Engl. J. Med. 2001; 345:1621-6.
- Moayeri M and Leppla SH. The roles of anthrax toxin in pathogenesis. Curr. Opin. Microbiol. 2004; 7:19-24.
- Bradley KA, Mogridge J, Mourez M, et al. Identification of the cellular receptor for anthrax toxin. Nature 2001; 414:225-9.
- Scobie HM, Rainey GJ, Bradley KA, et al. Human capillary morphogenesis protein 2 functions as an anthrax toxin receptor. Proc. Natl. Acad. Sci. USA 2003; 100:5170-4.
Buffer:
Anthrax Edema Factor Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Anthrax Edema Factor antibody was raised against a synthetic peptide corresponding to 16 amino acids near the carboxy terminus of the Anthrax edema factor protein.
The immunogen is located within the last 50 amino acids of Anthrax Edema Factor.
The immunogen is located within the last 50 amino acids of Anthrax Edema Factor.
NCBI Gene ID #:
3361726
NCBI Official Name:
Calmodulin-sensitive adenylate cyclase
NCBI Official Symbol:
pxo1_122
NCBI Organism:
Bacillus anthracis
Physical State:
Liquid
Protein Accession #:
P40136
Protein GI Number:
729244
Purification:
Anthrax Edema Factor Antibody is affinity chromatography purified via peptide column.
Research Area:
Infectious Disease
Swissprot #:
P40136
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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