Caspase-2 Antibody

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ProSci
Product Code: PSI-3447
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3447-0.02mg0.02mg£150.00
Quantity:
PSI-3447-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of Caspase-2 in (A) human thymus tissue and (B) human kidney lysate with Caspase-2 antibody at 1 μg/mL.
2 / 2
Immunocytochemistry of caspase-2 in A-20 cells with caspase-2 antibody at 2 μg/mL.
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Western blot analysis of Caspase-2 in (A) human thymus tissue and (B) human kidney lysate with Caspase-2 antibody at 1 μg/mL.
Immunocytochemistry of caspase-2 in A-20 cells with caspase-2 antibody at 2 μg/mL.

Documents

Further Information

Additional Names:
Caspase-2 Antibody: ICH1, NEDD2, CASP-2, NEDD-2, PPP1R57, ICH1, Caspase-2, Neural precursor cell expressed developmentally down-regulated protein 2
Application Note:
Caspase-2 antibody can be used for the detection of caspase-2 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL.

Antibody validated: Western Blot in human samples and Immunocytochemistry in mouse samples. All other applications and species not yet tested.
Background:
Caspase-2 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1β and IL-18. Although phylogenetically similar to this subfamily, Caspase-2 is thought to be involved in stress-induced apoptosis. Caspase-2 has two major isoforms; overexpression on the long form results in apoptosis while that of the short form suppresses cell death.
Background References:
  • Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004; 117:561-74.
  • Zhivotovsky B and Orrenius S. Caspase-2 funtion in response to DNA damage. Biochim. Biophys. Res. Comm. 2005; 331:859-67.
  • Kuida K, Lippke JA, Ku G, et al. Altered cytokine export and apoptosis in mice deficient in interleukin-1 β converting enzyme. Science 1995; 267:2000-3.
  • Gracie JA, Robertson SE, and McInnes IB. Interleukin-18. J. Leukoc. Biol. 2003; 73:213-224.
Buffer:
Caspase-2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (94%), Chicken: (75%)
Immunogen:
Caspase-2 antibody was raised against a 16 amino acid synthetic peptide from the amino-terminus of human Caspase-2.

The immunogen is located within the first 50 amino acids of Caspase-2.
NCBI Gene ID #:
835
NCBI Official Name:
caspase 2, apoptosis-related cysteine peptidase
NCBI Official Symbol:
CASP2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 51 kDa

Observed: 50 kDa
Protein Accession #:
P42575
Protein GI Number:
83300977
Purification:
Caspase-2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis,Cancer
SPECIFICITY:
Depending on cell lines or tissues used, other cleavage products may be observed.
Swissprot #:
P42575
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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