Caspase-4 Antibody

Product Code: PSI-3451

Supplier: ProSci

Code	Size	Price

PSI-3451-0.02mg

0.02mg

£150.00

Quantity:

PSI-3451-0.1mg

0.1mg

£449.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit

Antibody Isotype: IgG

Antibody Clonality: Polyclonal

Regulatory Status: RUO

Applications:

Enzyme-Linked Immunosorbent Assay (ELISA)
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Western Blot (WB)

Images

1 / 3

Western blot analysis of caspase-4 in Ramos cells with caspase-4 antibody at (A) 0.5 and (B) 1 μg/mL.

2 / 3

Immunohistochemical staining of mouse spleen using caspase-4 antibody at 2 μg/mL.

3 / 3

Immunofluorescence of Caspase-4 in Mouse Spleen 100 cells with Caspase-4 antibody at μg/mL.

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Documents

Further Information

Additional Names:

Caspase-4 Antibody: TX, ICH-2, Mih1/TX, ICEREL-II, ICE(rel)II, ICH2, Caspase-4, Protease ICH-2, CASP-4

Application Note:

Casp-4 antibody can be used for the detection of Caspase-4 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Background:

Caspase-4 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12. Activation of these caspases results in the cleavage and activation of proinflammatory cytokines such as IL-1β and IL-18. Caspase-4 was initially identified as a homologous protein to Caspase-1 and the C. elegans Ced-3 which could induce apoptosis in transfected cells. More recent studies have shown that it can be activated by ER stress and has been suggested to be involved in multiple neuronal pathologies such as Alzheimer's disease.

Background References:

Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004; 117:561-74.
Kuida K, Lippke JA, Ku G, et al. Altered cytokine export and apoptosis in mice deficient in interleukin-1 β converting enzyme. Science 1995; 267:2000-3.
Gracie JA, Robertson SE, and McInnes IB. Interleukin-18. J. Leukoc. Biol. 2003; 73:213-224.
Kamens J, Paskind M, Hugunin M, et al. Identification and characterization of ICH-2, a novel member of the interleukin-1 β-converting enzyme family of cysteine proteases. J. Biol. Chem. 1995; 270:15250-6.

Buffer:

Caspase-4 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration:

1 mg/mL

Conjugate:

Unconjugated

DISCLAIMER:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Immunogen:

Caspase-4 antibody was raised against a 16 amino acid synthetic peptide from the amino-terminus of human Caspase-4.

The immunogen is located within amino acids 20 - 70 of Caspase-4.

NCBI Gene ID #:

837

NCBI Official Name:

caspase 4, apoptosis-related cysteine peptidase

NCBI Official Symbol:

CASP4

NCBI Organism:

Homo sapiens

Physical State:

Liquid

Protein Accession #:

AAA86890

Protein GI Number:

886050

Purification:

Caspase-4 Antibody is affinity chromatography purified via peptide column.

Research Area:

Apoptosis,Cancer,Neuroscience

SPECIFICITY:

Depending on cell lines or tissues used, other cleavage products may be observed.

Swissprot #:

P49662

User NOte:

Optimal dilutions for each application to be determined by the researcher.

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