Caspase-5 Antibody

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ProSci
Product Code: PSI-3457
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3457-0.02mg0.02mg£150.00
Quantity:
PSI-3457-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of caspase-5 in Ramos cells with caspase-5 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
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Immunocytochemistry of caspase-5 in P815 cells with caspase-5 antibody at 2 μg/mL.
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Immunofluorescence of Caspase-5 in P815 cells with Caspase-5 antibody at 2 μg/mL.
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Western blot analysis of caspase-5 in Ramos cells with caspase-5 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
Immunocytochemistry of caspase-5 in P815 cells with caspase-5 antibody at 2 μg/mL.
Immunofluorescence of Caspase-5 in P815 cells with Caspase-5 antibody at 2 μg/mL.

Documents

Further Information

Additional Names:
Caspase-5 Antibody: ICH-3, ICEREL-III, ICE(rel)III, ICH3, Caspase-5, Protease ICH-3, CASP-5
Application Note:
Casp-5 antibody can be used for the detection of caspase-5 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 2 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
Caspase-5 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12. Activation of these caspases results in the cleavage and activation of proinflammatory cytokines such as IL-1β and IL-18. Caspase-5 can interact with caspase-1; both are constituents of the NALP1 inflammasome, a complex that can trigger the cleavage of pro-IL-1β. Expression of caspase-5 can be regulated by lipopolysaccharide (LPS) and IFN-gamma.
Background References:
  • Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004; 117:561-74.
  • Kuida K, Lippke JA, Ku G, et al. Altered cytokine export and apoptosis in mice deficient in interleukin-1 β converting enzyme. Science 1995; 267:2000-3.
  • Gracie JA, Robertson SE, and McInnes IB. Interleukin-18. J. Leukoc. Biol. 2003; 73:213-224.
  • Martinon F, Burns K, and Tschopp J. The inflammasome: a molecular platform triggering activation of the inflammatory caspases and processing of proIL-1beta. Mol. Cell. 2002; 10:417-26.
Buffer:
Caspase-5 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (79%)
Immunogen:
Caspase-5 antibody was raised against a 14 amino acid synthetic peptide from the center of human Caspase-5.

The immunogen is located within amino acids 170 - 220 of Caspase-5.
NCBI Gene ID #:
838
NCBI Official Name:
caspase 5, apoptosis-related cysteine peptidase
NCBI Official Symbol:
CASP5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
P51878
Protein GI Number:
212276423
Purification:
Caspase-5 Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis
SPECIFICITY:
Depending on cell lines or tissues used, other cleavage products may be observed.
Swissprot #:
P51878
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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