TOR Antibody

Contact us
ProSci
Product Code: PSI-3485
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3485-0.02mg0.02mg£150.00
Quantity:
PSI-3485-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

1 / 2
Western blot analysis of TOR in L1210 cell lysate with TOR antibody at (A) 1 and (B) 2 μg/mL.
2 / 2
Immunocytochemistry of TOR in L1210 cells with TOR antibody at 2 μg/mL.
❮❯

Western blot analysis of TOR in L1210 cell lysate with TOR antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of TOR in L1210 cells with TOR antibody at 2 μg/mL.

Documents

Further Information

Additional Names:
TOR Antibody: FRAP, FRAP1, FRAP2, RAFT1, RAPT1, FRAP, FK506-binding protein 12-rapamycin complex-associated protein 1, mTOR
Application Note:
TOR antibody can be used for the detection of TOR by Western blot at 1 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL.

Antibody validated: Western Blot in mouse samples and Immunocytochemistry in mouse samples. All other applications and species not yet tested.
Background:
TOR Antibody: The mammalian Target of Rapamycin (TOR, also known as mTOR) is an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. It was initially discovered as a kinase whose ability to stimulate T cell proliferation in response to IL-2 could be inhibited by the immunosuppressive drug rapamycin. Rapamycin inhibits TOR in other cell types resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation. TOR is normally associated with the regulatory proteins RAPTOR and GbetaL. Its downstream targets are thought to be the ribosomal protein S6 kinases and the eukaryotic initiation factor 4E binding proteins (4EBPs). Regulation of these protein families allows TOR to control protein biosynthesis.
Background References:
  • Shamji AF, Ngheim P, and Schreiber SL. Integration of growth factor and nutrient signaling: implications for cancer biology. Mol. Cell 2003; 12:271-80.
  • Sabatini DM, Erdjument-Bromage H, Lui M, et al. RAFT1: a mammalian protein that binds to FKP12 in a rapamycin-dependent fashion and is homologous to yeast TORs. Cell 1994; 78:35-43.
  • Cardenas ME, Zhu D, and Heitman J. Molecular mechanisms of immunosuppression by cyclosporine, FK506, and rapamycin. Curr. Opin. Nephrol. Hypertens. 1995; 4:472-7.
  • Fingar DC and Blenis J. Target of rapamycin (TOR): an integrator of nutrient and growth factor signals and coordinator of cell growth and cell cycle progression. Oncogene 2004; 23:3151-71.
Buffer:
TOR Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (100%)
Immunogen:
TOR antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human TOR.

The immunogen is located within amino acids 210 - 260 of TOR.
NCBI Gene ID #:
2475
NCBI Official Name:
mechanistic target of rapamycin
NCBI Official Symbol:
MTOR
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004949
Protein GI Number:
4826730
Purification:
TOR Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction,Neuroscience
Swissprot #:
P42345
User NOte:
Optimal dilutions for each application to be determined by the researcher.

Related Products

Product NameProduct CodeSupplier 
TOR PeptidePSI-3485PProSci Summary Details