Raptor Antibody

Product Code: PSI-3489

Supplier: ProSci

Code	Size	Price

PSI-3489-0.02mg

0.02mg

£150.00

Quantity:

PSI-3489-0.1mg

0.1mg

£449.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit

Antibody Isotype: IgG

Antibody Clonality: Polyclonal

Regulatory Status: RUO

Applications:

Enzyme-Linked Immunosorbent Assay (ELISA)
Immunohistochemistry (IHC)
Western Blot (WB)

Images

1 / 2

Western blot analysis of Raptor in L1210 cell lysate with Raptor antibody at (A) 2 and (B) 4 μg/mL.

2 / 2

Immunocytochemistry of Raptor in L1210 cells with Raptor antibody at 10 μg/mL.

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Documents

Further Information

Additional Names:

Raptor Antibody: Rap, Raptor, mKIAA1303, 4932417H02Rik, Regulatory-associated protein of mTOR

Application Note:

Raptor antibody can be used for the detection of Raptor by Western blot at 2 and 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.

Antibody validated: Western Blot in mouse samples and Immunocytochemistry in mouse samples. All other applications and species not yet tested.

Background:

Raptor Antibody: The mammalian Target of Rapamycin (TOR, also known as mTOR) is an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. Rapamycin inhibits TOR activity resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation. Raptor (regulatory associated protein of TOR) is a TOR-binding protein essential for TOR signaling in vivo. It acts as a TOR scaffold protein whose binding by TOR substrates is necessary for effective TOR-catalyzed phosphorylation. These substrates include the ribosomal protein S6 kinase (RP S6K) and the eukaryotic initiation factor 4E binding protein 4EBP1, proteins necessary for cell growth and proliferation and responsive to nutrient and mitogen levels. Raptor binds these proteins through a common 5 amino acid TOR-signaling (TOS) motif; mutation of this motif prevents the TOR-dependent phosphorylation of these proteins.

Background References:

Shamji AF, Ngheim P, and Schreiber SL. Integration of growth factor and nutrient signaling: implications for cancer biology. Mol. Cell 2003; 12:271-80.
Fingar DC and Blenis J. Target of rapamycin (TOR): an integrator of nutrient and growth factor signals and coordinator of cell growth and cell cycle progression. Oncogene 2004; 23:3151-71.
Yonezawa K, Tokunaga C, Oshiro N, et al. Raptor, a binding partner of target of rapamycin. Biochem. Biophys. Res. Commun. 2004; 313:437-441.
Hara K, Yonezawa K, Weng QP, et al. Amino acid sufficiency and mTOR regulate p70 S6 kinase and eIF-4E BP1 through a common effector mechanism. J. Biol. Chem. 1998; 273:14484-94.

Buffer:

Raptor Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration:

1 mg/mL

Conjugate:

Unconjugated

DISCLAIMER:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Homology:

Predicted species reactivity based on immunogen sequence: Human: (87%)

Immunogen:

Raptor (CT) polyclonal antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of mouse Raptor.

The immunogen is located within amino acids 1140 - 1190 of Raptor.

NCBI Gene ID #:

74370

NCBI Official Name:

regulatory associated protein of MTOR, complex 1

NCBI Official Symbol:

Rptor

NCBI Organism:

Mus musculus

Physical State:

Liquid

Protein Accession #:

Q8K4Q0

Protein GI Number:

46577497

Purification:

Raptor Antibody is affinity chromatography purified via peptide column.

Research Area:

Signal Transduction,Neuroscience

SPECIFICITY:

Raptor has multiple isoforms that may also be recognized by antibody.

Swissprot #:

Q8K4Q0

User NOte:

Optimal dilutions for each application to be determined by the researcher.

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