Rheb Antibody

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ProSci
Product Code: PSI-3499
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3499-0.02mg0.02mg£150.00
Quantity:
PSI-3499-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)

Images

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Western blot analysis of Rheb in rat heart cell lysate with Rheb antibody at (A) 2 and (B) 4 μg/mL.
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Western blot analysis of Rheb in rat heart cell lysate with Rheb antibody at (A) 2 and (B) 4 μg/mL.

Documents

Further Information

Additional Names:
Rheb Antibody: RHEB2, RHEB2, GTP-binding protein Rheb, Ras homolog enriched in brain
Application Note:
Rheb antibody can be used for the detection of Rheb by Western blot at 2 and 4 μg/mL.

Antibody validated: Western Blot in rat samples. All other applications and species not yet tested.
Background:
Rheb Antibody: Rheb (Ras homolog enriched in brain) is an evolutionarily conserved member of the Ras family of small GTP-binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure. While it is expressed at relatively high levels in the brain, Rheb is widely expressed in other tissues and may be induced by growth factor stimulation. Similar to other family members, Rheb triggers activation of the Raf-MEK-MAPK pathway. Biochemical and genetic studies demonstrate that Rheb has an important role in regulating the insulin/Target of rapamycin (TOR) signaling pathway. TOR is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with protein translation and cell growth. A dimeric protein complex termed TSC1/TSC2 indirectly inhibits TOR activity by inhibiting Rheb via the GAP activity of TSC2.
Background References:
  • Yamagata K, Sanders LK, Kaufman WE, et al. rheb, a growth factor- and synaptic activity-regulated gene, encodes a novel Ras-related protein. J. Biol. Chem. 1994; 269:16333-9.
  • Yee WM and Worley PF. Rheb interacts with Raf-1 kinase and may function to integrate growth factor- and protein kinase A-dependent signals. Mol. Cell. Biol. 1997; 17:921-3.
  • Inoki K, Li Y, Xu T, et al. Rheb GTPase is a direct target of TSC2 GAP activity and regulates mTOR signaling. Genes Dev. 2003; 17:1829-34.
  • Stocker H, Radimerski T, Schindelholz B, et al. Rheb is an essential regulator of S6K in controlling cell growth in Drosophila. Nat. Cell Biol. 2003; 5:559-65.
Buffer:
Rheb Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
Rabbit Rheb polyclonal antibody was raised against a 14 amino acid synthetic peptide from the amino terminus of human Rheb.

The immunogen is located within the first 50 amino acids of Rheb.
NCBI Gene ID #:
6009
NCBI Official Name:
Ras homolog enriched in brain
NCBI Official Symbol:
RHEB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
AAH16155
Protein GI Number:
16740561
Purification:
Rheb Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction,Cancer
SPECIFICITY:
Post-translationally modified Rheb is sometimes observed at higher molecular weight.
Swissprot #:
Q15382
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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