TSC2 Antibody

Product Code: PSI-3507

Supplier: ProSci

Code	Size	Price

PSI-3507-0.02mg

0.02mg

£150.00

Quantity:

PSI-3507-0.1mg

0.1mg

£449.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit

Antibody Isotype: IgG

Antibody Clonality: Polyclonal

Regulatory Status: RUO

Applications:

Enzyme-Linked Immunosorbent Assay (ELISA)
Western Blot (WB)

Images

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Western blot analysis of TSC2 in L1210 cell lysate with TSC2 antibody at 1 μg/mL in the (A) absence and (B) presence of blocking peptide.

2 / 2

Western blot analysis of TSC2 in Jurkat cell lysate with TSC2 antibody at (A) 1 and (B) 2 μg/mL..

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Documents

Further Information

Additional Names:

TSC2 Antibody: LAM, TSC4, Tuberin, Tuberous sclerosis 2 protein

Application Note:

TSC2 antibody can be used for the detection of TSC2 by Western blot at 1 - 2 μg/mL.

Antibody validated: Western Blot in human and mouse samples. All other applications and species not yet tested.

Background:

TSC2 Antibody: Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high. Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro, suggesting that the TSC1/TSC2 complex decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing that overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth. At least three isoforms of TSC2 exist.

Background References:

Shamji AF, Ngheim P, and Schreiber SL. Integration of growth factor and nutrient signaling: implications for cancer biology. Mol. Cell 2003; 12:271-80.
Inoki K, Ouyang H, Li Y, et al. Signaling by target of rapamycin proteins in cell growth control. Microbiol. Mol. Biol. Rev. 2005; 69:79-100.
Tabancay Jr AP, Gau CL, Machado IM, et al. Identification of dominant negative mutants of Rheb GTPase and their use to implicate the involvement of human Rheb in the activation of p70S6K. J. Biol. Chem. 2003; 278:39921-30.
Inoki K, Li Y, Xu T, et al. Rheb GTPase is a direct target of TSC2 GAP activity and regulates mTOR signaling. Genes Dev. 2003; 17:1829-34.

Buffer:

TSC2 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration:

1 mg/mL

Conjugate:

Unconjugated

DISCLAIMER:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Homology:

Predicted species reactivity based on immunogen sequence: Rat: (86%)

Immunogen:

TSC2 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human TSC2.

The immunogen is located within amino acids 1450 - 1500 of TSC2.

NCBI Gene ID #:

7249

NCBI Official Name:

tuberous sclerosis 2

NCBI Official Symbol:

TSC2

NCBI Organism:

Homo sapiens

Physical State:

Liquid

PREDICTED MOLECULAR WEIGHT:

Predicted: 200 kDa

Observed: 200 kDa

Protein Accession #:

NP_000539

Protein GI Number:

116256352

Purification:

TSC2 Antibody is affinity chromatography purified via peptide column.

Research Area:

Signal Transduction

Swissprot #:

P49815

User NOte:

Optimal dilutions for each application to be determined by the researcher.

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