RPS6K1 Antibody

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ProSci
Product Code: PSI-3511
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3511-0.02mg0.02mg£150.00
Quantity:
PSI-3511-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of RPS6K1 in Jurkat cell lysate with RPS6K1 antibody at (A) 2.5, (B) 5 and (C) 10 μg/mL.
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Immunocytochemistry of RPS6K1 in Jurkat cells with RPS6K1 antibody at 10 μg/mL.
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Immunofluorescence of RP S6 Kinase in Jurkat cells with RP S6 Kinase antibody at 20 μg/mL.
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Western blot analysis of RPS6K1 in Jurkat cell lysate with RPS6K1 antibody at (A) 2.5, (B) 5 and (C) 10 μg/mL.
Immunocytochemistry of RPS6K1 in Jurkat cells with RPS6K1 antibody at 10 μg/mL.
Immunofluorescence of RP S6 Kinase in Jurkat cells with RP S6 Kinase antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
RPS6K1 Antibody: RSK, HU-1, RSK1, MAPKAPK1A, Ribosomal protein S6 kinase alpha-1, 90 kDa ribosomal protein S6 kinase 1, S6K-alpha-1
Application Note:
RPS6K1antibody can be used for the detection of RPS6K1 by Western blot at 2.5 - 10 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
RPS6K1 Antibody: Ribosomal protein S6 kinase 1 (RPS6K1) is the best characterized effector of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. Nutrients and growth factors stimulate a complex including TOR, raptor (regulatory associated protein of TOR), and GbetaL to phosphorylate RPS6K1 and the eukaryotic initiation factor 4E binding protein (4EBP1), leading to increased protein synthesis and cell growth. RPS6K1 is thought to desensitize tissues to insulin as mice deficient in RPS6K1 have been shown to be hypersensitive to insulin and impervious to obesity-induced insulin resistance seen in wild type obese mice.
Background References:
  • Burnett PE, Barrow RK, Cohen NA, et al. RAFT1 phosphorylation of the translational regulators p70 S6 kinase and 4E-BP1. Proc. Natl. Acad. Sci. USA. 1998; 95:1432-7.
  • Inoki K, Ouyang H, Li Y, et al. Signaling by target of rapamycin proteins in cell growth control. Microbiol. Mol. Biol. Rev. 2005; 69:79-100.
  • Manning BD. Balancing Akt with S6K: implications for both metabolic diseases and tumorigenesis. J. Cell Biol. 2004; 167:399-403.
  • Um SH, Frigerio F, Watanabe M, et al. Absences of S6K1 protects against age- and diet-induced obesity while enhancing insulin sensitivity. Nature 2004; 431:200-5.
Buffer:
RPS6K1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (100%), Chicken: (100%), Mouse: (100%)
Immunogen:
RPS6K1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human RPS6K1.

The immunogen is located within amino acids 600 - 650 of RPS6K1.
NCBI Gene ID #:
6195
NCBI Official Name:
ribosomal protein S6 kinase, 90kDa, polypeptide 1
NCBI Official Symbol:
RPS6KA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
AAC82497
Protein GI Number:
292457
Purification:
RPS6K1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
Swissprot #:
Q15418
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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