CTRP3 Antibody

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ProSci
Product Code: PSI-3565
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3565-0.02mg0.02mg£150.00
Quantity:
PSI-3565-0.1mg0.1mg£449.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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<strong>Figure 1 Western Blot Validation in Human and Mouse Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: CTRP3, 3565 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 2 Western Blot Validation with Recombinant Protein</strong><br> Loading: 30 ng of human CTRP3 recombinant protein per lane. Antibodies: CTRP3, 3565 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 3 Western Blot Validation in Mouse and Rat Tissue Lysates</strong><br> Loading: 15 μg of lysates per lane. Antibodies: CTRP3, 3565 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 4 Western Blot Validation in Mouse Heart Tissue Lysate in the (A) absence and (B) presence of blocking peptide</strong><br> Loading: 15 μg of lysates per lane. Antibodies: CTRP3, 3565 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 5 Immunohistochemistry Validation of CTRP3 in Mouse Heart Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-CTRP3 antibody (3565) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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<strong>Figure 6 Immunofluorescence Validation of CTRP3 in Mouse Heart Tissue</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse heart tissue labeling CTRP3 with 3565 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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<strong>Figure 1 Western Blot Validation in Human and Mouse Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: CTRP3, 3565 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Western Blot Validation with Recombinant Protein</strong><br> Loading: 30 ng of human CTRP3 recombinant protein per lane. Antibodies: CTRP3, 3565 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation in Mouse and Rat Tissue Lysates</strong><br> Loading: 15 μg of lysates per lane. Antibodies: CTRP3, 3565 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Western Blot Validation in Mouse Heart Tissue Lysate in the (A) absence and (B) presence of blocking peptide</strong><br> Loading: 15 μg of lysates per lane. Antibodies: CTRP3, 3565 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 5 Immunohistochemistry Validation of CTRP3 in Mouse Heart Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-CTRP3 antibody (3565) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 6 Immunofluorescence Validation of CTRP3 in Mouse Heart Tissue</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse heart tissue labeling CTRP3 with 3565 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Documents

Further Information

Additional Names:
CTRP3 Antibody: CORS, CORCS, CTRP3, CORS26, C1ATNF3, CORS-26, UNQ753/PRO1484, Complement C1q tumor necrosis factor-related protein 3, Collagenous repeat-containing sequence 26 kDa protein
Application Note:
WB: 1 μg/mL; IHC: 2 μg/mL; IF: 10 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in mouse samples; Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
CTRP3 Antibody: Adipose tissue of an organism plays a major role in regulating physiologic and pathologic processes such as metabolism and immunity by producing and secreting a variety of bioactive molecules termed adipokines. One highly conserved family of adipokines is adiponectin/ACRP30 and its structural and functional paralogs, the C1q/tumor necrosis factor-alpha-related proteins (CTRPs) 1-7. Unlike adiponectin, which is expressed exclusively by differentiated adipocytes, the CTRPs are expressed in a wide variety of tissues. An analysis of the crystal structure of adiponectin revealed a structural and evolutionary link between TNF and C1q-containing proteins, suggesting that these proteins arose from a common ancestral innate immunity gene. It has been suggested that CTRP3 may play a role in skeletal development.
Background References:
  • Fantuzzi. J. Allergy Clin. Immunol. 2005; 115:911-9.
  • Tsao et al. Euro. J. Pharmacol. 2002; 440:213-21.
  • Wong et al. Proc. Natl. Acad. Sci. USA 2004; 101:10302-7.
  • Shapiro and Scherer. Curr. Biol. 1998; 8:335-8.
Buffer:
CTRP3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Anti-CTRP3 antibody (3565) was raised against a peptide corresponding to 16 amino acids near the center of human CTRP3.

The immunogen is located within amino acids 90-140 of CTRP3.
ISOFORMS:
Human CTRP3 has 3 isoforms, including isoform 1 (246aa, 27kD), isoform 2 (198aa, 22kD) and isoform 3 (319aa, 35kD). Mouse CTRP3 has one isoform (246aa, 27kD) and Rat CTRP3 also has one isoform (319aa, 35kD). 3565 can detect human, mouse and rat.
NCBI Gene ID #:
114899
NCBI Official Name:
C1q and tumor necrosis factor related protein 3
NCBI Official Symbol:
C1QTNF3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 27kD

Observed: 30 kD (Post-modyfication: 1 N-linked glycosylation)
Protein Accession #:
NP_852100
Protein GI Number:
62739183
Purification:
CTRP3 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
SPECIFICITY:
Multiple isoforms of CTRP3 are known to exist. CTRP3 antibody is predicted to not recognize other members of the CTRP protein family.
Swissprot #:
Q9BXJ4
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:

Recombinant Protein Test (Figure 2): Anti-CTRP3 antibodies (3565) detected human CTRP3 recombinant protein.

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