Precerebellin Antibody

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ProSci
Product Code: PSI-3589
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3589-0.02mg0.02mg£150.00
Quantity:
PSI-3589-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of precerebellin in mouse cerebellum lysate with precerebellin antibody at (A) 2 and (B) 4 μg/mL.
2 / 2
Immunohistochemical staining of rat brain tissue using precerebellin antibody at 2 μg/mL.
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Western blot analysis of precerebellin in mouse cerebellum lysate with precerebellin antibody at (A) 2 and (B) 4 μg/mL.
Immunohistochemical staining of rat brain tissue using precerebellin antibody at 2 μg/mL.

Documents

Further Information

Additional Names:
Precerebellin Antibody: Cerebellin-1, Precerebellin, CER
Application Note:
Precerebellin antibody can be used for the detection of precerebellin by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL.

Antibody validated: Western Blot in mouse samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
Background:
Precerebellin Antibody: Precerebellin is the precursor of the brain-specific hexadecapeptide cerebellin, a protein with substantial similarity to the globular region of the B chain of complement component C1q. Cerebellin exerts neuromodulatory functions by directly stimulating norepinephrine release via the adenylate cyclase/pka- dependent signaling pathway; and indirectly enhances adrenocortical secretion in vivo, through a paracrine mechanism involving medullary catecholamine release. The active form of precerebellin is highly enriched in postsynaptic structures of cerebellar Purkinje cells in cartwheel neurons of the dorsal cochlear nucleus. Because precerebellin belongs to the C1q/tumor necrosis factor superfamily of secreted proteins and has similarity to adiponectin and CTRP3, it has been suggested that precerebellin posseses functions other than those already stated.
Background References:
  • Urade Y, Oberdick J, Molinar-Rode R, et al. Precerebellin is a cerebellum-specific protein with similarity to the globular component C1q B chain. Proc. Natl. Acad. Sci. USA1991; 88:1069-73.
  • Mazzocchi G, Andreis PG, De Caro R, et al. Cerebellin enhances in vitro secretory activity of human adrenal gland. J. Clin. Endocrinol. Metab. 1999; 84:632-5.
  • Albertin G, Malendowicz LK, Macchi C, et al. Cerebellin stimulates the secretory activity of the rat adrenal gland: in vitro and in vivo studies. Neuropeptides 2000; 34:7-11.
  • Shamji AF, Ngheim P, and Schreiber SL. Integration of growth factor and nutrient signaling: implications for cancer biology. Proc. Natl. Acad. Sci. USA 1985; 82:7145-8.
Buffer:
Precerebellin Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
Precerebellin antibody was raised against a 15 amino acid synthetic peptide from the middle of human precerebellin.

The immunogen is located within amino acids 50 - 100 of Precerebellin.
NCBI Gene ID #:
869
NCBI Official Name:
cerebellin 1 precursor
NCBI Official Symbol:
CBLN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
P23435
Protein GI Number:
116114
Purification:
Precerebellin Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction,Neuroscience
Swissprot #:
P23435
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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