GITRL Antibody

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ProSci
Product Code: PSI-3591
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3591-0.02mg0.02mg£150.00
Quantity:
PSI-3591-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of (A) 5 ng, (B) 25 ng, and (C) 50 ng of purified recombinant GITRL with ITRL antibody at 1 μg/mL.
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Immunocytochemistry of GITRL in THP-1 cells with GITRL antibody at 10 μg/mL.
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Western blot analysis of (A) 5 ng, (B) 25 ng, and (C) 50 ng of purified recombinant GITRL with ITRL antibody at 1 μg/mL.
Immunocytochemistry of GITRL in THP-1 cells with GITRL antibody at 10 μg/mL.

Documents

Further Information

Additional Names:
GITRL Antibody: TL6, AITRL, GITRL, hGITRL, TL6, UNQ149/PRO175, Tumor necrosis factor ligand superfamily member 18, Activation-inducible TNF-related ligand
Application Note:
GITRL antibody can be used for the detection of GITRL by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.

Antibody validated: Immunocytochemistry in human samples. All other applications and species not yet tested.
Background:
GITRL Antibody: The tumor necrosis factor (TNF) and TNF receptor (TNFR) gene superfamilies regulate numerous biological functions including cell proliferation, differentiation, and survival through regulating the activation of the transcription factor NF-κB and various mitogen-activated protein kinases. The glucocorticoid-induced tumor necrosis factor receptor (GITR) is an emerging member of this family that is expressed on CD4+ CD25+ regulatory T cells and appears to have crucial immune regulation functions. Its ligand GITRL is expressed in endothelial and antigen-presenting cells and can activate NF-κB, induce both pro- and anti-apoptotic effects, inhibit the suppressive activity of regulatory T cells, and co-stimulate responder T cells through GITR. Dominant negative forms of NIK and TRAF2 expressed in transfected 293 cells substantially inhibited NF-κB activation, suggesting that the GITRL-GITR pathway involves both NIK and TRAF2.
Background References:
  • Gaur U, Aggarwal BB. Regulation of proliferation, survival and apoptosis by members of the TNF superfamily. Biochem. Pharmacol. 2003; 66:1403-8.
  • Ronchetti S, Nocentini G, Riccardi C, et al. Role of GITR in activation response of T lymphocytes.Blood 2002; 100:350-2.
  • Shimizu J, Yamakai S, Takahashi T, et al. Stimulation of CD25(+) CD4(+) regulatory T cells through GITR breaks immunological self- tolerance. Nat. Immunol. 2002;3:135-42.
  • Gurney AL, Marsters SA, Huang A, et al. Identification of a new member of the tumor necrosis factor family and its receptor, a human ortholog of mouse GITR. Curr. Biol. 1999; 9:215-218.
Buffer:
GITRL Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
GITRL antibody was raised against purified recombinant human GITR ligand.
NCBI Gene ID #:
8995
NCBI Official Name:
tumor necrosis factor (ligand) superfamily, member 18
NCBI Official Symbol:
TNFSF18
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
Q9UNG2
Protein GI Number:
13124621
Purification:
GITRL Antibody is Protein A purified.
Research Area:
Chemokines & Cytokines
Swissprot #:
Q9UNG2
User NOte:
Optimal dilutions for each application to be determined by the researcher.