IRAK2 Antibody

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ProSci
Product Code: PSI-3595
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3595-0.02mg0.02mg£150.00
Quantity:
PSI-3595-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of IRAK2 in RAW264.7 whole cell lysate with mIRAK2 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
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Immunocytochemistry of IRAK2 in A-20 cells with IRAK2 antibody at 1 μg/mL.
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Immunofluorescence of IRAK2 in A20 cells with IRAK2 antibody at 10 μg/mL.
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Immunofluorescence of IRAK-2 in Hela cells with IRAK 2 antibody at 20 μg/mL.<br><br>Red: IRAK2 Antibody (3595) <br> Blue: DAPI staining
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Western blot analysis of IRAK2 in RAW264.7 whole cell lysate with mIRAK2 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
Immunocytochemistry of IRAK2 in A-20 cells with IRAK2 antibody at 1 μg/mL.
Immunofluorescence of IRAK2 in A20 cells with IRAK2 antibody at 10 μg/mL.
Immunofluorescence of IRAK-2 in Hela cells with IRAK 2 antibody at 20 μg/mL.<br><br>Red: IRAK2 Antibody (3595) <br> Blue: DAPI staining

Documents

Further Information

Additional Names:
IRAK2 Antibody: IRAK-2, AI649099, 6330415L08Rik, Interleukin-1 receptor-associated kinase-like 2, IRAK-2
Application Note:
IRAK2 antibody can be used for detection of IRAK2 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
Background:
IRAK2 Antibody: The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-κB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family has been identified and designated IRAK2. Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-κB activation. IRAKs also associate with Toll like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kB activation. Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. Unlike human IRAK2, murine IRAK2 exists as four alternately spliced isoforms (IRAK2a-d), with two isoforms (IRAK2c and d) acting in an inhibitory fashion. IRAK2 is expressed in a variety of tissues.
Background References:
  • Muzio M, Ni J, Feng P, et al. IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling. Science 1997; 278:1612-5.
  • Zhang FX, Kirschning CJ, Mancinelli R, et al. Bacterial lipopolysaccharide activates nuclear factor-κB through interleukin-1 signaling mediators in cultured human dermal endothelial cells and mononuclear phagocytes. J. Biol. Chem. 1999; 274:7611-4.
  • Yang RB, Mark MR, Gurney AL, et al. Signaling events induced by lipopolysaccharide-activated toll-like receptor 2. J. Immunol. 1999; 163:639-43.
  • Hardy MP and O'Neill LAJ. The murine IRAK2 gene encodes four alternately spliced isoforms, two of which are inhibitory. J. Biol. Chem. 2004; 279:27699-708.
Buffer:
IRAK2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (88%), Bovine: (82%)
Immunogen:
IRAK2 antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of mouse IRAK2a.

The immunogen is located within the last 50 amino acids of IRAK2.
NCBI Gene ID #:
108960
NCBI Official Name:
interleukin-1 receptor-associated kinase 2
NCBI Official Symbol:
Irak2
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 68 kDa

Observed: 65 kDa
Protein Accession #:
AAO24761
Protein GI Number:
37725373
Purification:
IRAK2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction,
SPECIFICITY:
At least four isoforms of IRAK2 are known to exist; this antibody will detect all four isoforms. Anti-IRAK2 has no cross response to IRAK.
Swissprot #:
Q8CFA1
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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