IRAK2 Antibody

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ProSci
Product Code: PSI-3597
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3597-0.02mg0.02mg£150.00
Quantity:
PSI-3597-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)

Images

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Western blot analysis of IRAK2 in A-20 whole cell lysate with IRAK2 antibody (C2) at (A) 0.5, (B) 1, and (C) 2 μg/mL.
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Western blot analysis of IRAK2 in A-20 whole cell lysate with IRAK2 antibody (C2) at (A) 0.5, (B) 1, and (C) 2 μg/mL.

Documents

Further Information

Additional Names:
IRAK2 Antibody: IRAK-2, AI649099, 6330415L08Rik, Interleukin-1 receptor-associated kinase-like 2, IRAK-2
Application Note:
IRAK2 antibody can be used for detection of mouse IRAK2 by Western blot at 0.5 - 2 μg/mL.

Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Background:
IRAK2 Antibody: The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-κB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family has been identified and designated IRAK2. Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-κB activation. IRAKs also associate with Toll like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-κB activation. Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. Unlike human IRAK2, murine IRAK2 exists as four alternately spliced isoforms (IRAK2a-d), with two isoforms (IRAK2c and d) acting in an inhibitory fashion. IRAK2 is expressed in a variety of tissues.
Background References:
  • Muzio M, Ni J, Feng P, et al. IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling. Science 1997; 278:1612-5.
  • Zhang FX, Kirschning CJ, Mancinelli R, et al. Bacterial lipopolysaccharide activates nuclear factor-κB through interleukin-1 signaling mediators in cultured human dermal endothelial cells and mononuclear phagocytes. J. Biol. Chem. 1999; 274:7611-4.
  • Yang RB, Mark MR, Gurney AL, et al. Signaling events induced by lipopolysaccharide-activated toll-like receptor 2. J. Immunol. 1999; 163:639-43.
  • Hardy MP and O'Neill LAJ. The murine IRAK2 gene encodes four alternately spliced isoforms, two of which are inhibitory. J. Biol. Chem. 2004; 279:27699-708.
Buffer:
IRAK2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (94%), Rat: (94%), Human: (81%)
Immunogen:
RAK2 antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of mouse IRAK2a which is common to all four isoforms.

The immunogen is located within the last 50 amino acids of IRAK2.
NCBI Gene ID #:
108960
NCBI Official Name:
interleukin-1 receptor-associated kinase 2
NCBI Official Symbol:
Irak2
NCBI Organism:
Mus musculus
Physical State:
Liquid
Protein Accession #:
AAO24761
Protein GI Number:
37725373
Purification:
IRAK2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction,Neuroscience
SPECIFICITY:
Anti-IRAK2 has no cross response to IRAK.
Swissprot #:
Q8CFA1
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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