TRAF3 Antibody

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ProSci
Product Code: PSI-3601
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3601-0.02mg0.02mg£150.00
Quantity:
PSI-3601-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of TRAF3 in HeLa cell lysate with TRAF3 antibody at (A) 2 and (B) 4 μg/mL.
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Immunocytochemistry of TRAF3 in HeLa cells with TRAF3 antibody at 10 μg/mL.
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Western blot analysis of TRAF3 in HeLa cell lysate with TRAF3 antibody at (A) 2 and (B) 4 μg/mL.
Immunocytochemistry of TRAF3 in HeLa cells with TRAF3 antibody at 10 μg/mL.

Documents

Further Information

Additional Names:
TRAF3 Antibody: CAP1, LAP1, CAP-1, CRAF1, IIAE5, CD40bp, CAP1, TNF receptor-associated factor 3
Application Note:
TRAF3 antibody can be used for the detection of TRAF3 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.

Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Background:
TRAF3 Antibody: Tumor necrosis factor (TNF) receptor associated factors (TRAFs) are the major signal transducers for the TNF receptor superfamily and the interleukin-1 receptor/Toll-like receptor (IL-1/TLR) superfamily. TRAF3 was first identified by its interaction with CD40 and the Epstein-Barr virus transforming protein LMP1. Several TRAF3 mRNA splice variants exist and some of these can activate the transcription factor NF-κB. Besides CD40, TRAF3 also interacts with the TRFR superfamily member lymphotoxin-beta receptor (LTbetaR) in association with TRAF2 and the apoptosis inhibitors cIAP1 and Smac. It has been suggested that TRAF3 induces mitochondria-mediated apoptosis upon binding of the TNF family cytokine LIGHT by LTbetaR.
Background References:
  • Arch RH, Gedrich RW, and Thompson CB. Tumor necrosis factor receptor-associated factors (TRAFs) - a family of adaptor proteins that regulate life and death.Genes Dev. 1998; 12:2821-30.
  • Cheng G, Cleary AM, Ye Z, et al. Involvement of CRAF1, a relative of TRAF, in CD40 signaling. Science 1995; 267:1494-8.
  • Mosialos G, Birkenbach M, Yalamanchili R, et al. The Epstein-Barr virus transforming protein LMP1 engages signaling proteins for the tumor necrosis factor receptor family. Cell 1995; 80:389-99.
  • van Eyndhoven WG, Gamper CJ, Cho E, et al. TRAF-3 mRNA splice-deletion variants encode isoforms that induce NF0κB activation. Mol. Immunol. 1999; 36:647-58.
Buffer:
TRAF3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
TRAF3 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human.

The immunogen is located within amino acids 20 - 70 of TRAF3.
NCBI Gene ID #:
7187
NCBI Official Name:
TNF receptor-associated factor 3
NCBI Official Symbol:
TRAF3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_663777
Protein GI Number:
22027618
Purification:
TRAF3 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction,Neuroscience,Cancer
Swissprot #:
Q13114
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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