AES Antibody

Contact us
ProSci
Product Code: PSI-3609
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3609-0.02mg0.02mg£150.00
Quantity:
PSI-3609-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

1 / 3
Western blot analysis of AES in 293 cell lysate with AES antibody at (A) 2 and (B) 4 μg/mL.
2 / 3
Immunocytochemistry of AES in 293 cells with AES antibody at 10 μg/mL.
3 / 3
Immunofluorescence of AES in 293 cells with AES antibody at 20 μg/mL.
❮❯

Western blot analysis of AES in 293 cell lysate with AES antibody at (A) 2 and (B) 4 μg/mL.
Immunocytochemistry of AES in 293 cells with AES antibody at 10 μg/mL.
Immunofluorescence of AES in 293 cells with AES antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
AES Antibody: GRG, ESP1, GRG5, TLE5, AES-1, AES-2, GRG, Amino-terminal enhancer of split, Gp130-associated protein GAM, Amino enhancer of split
Application Note:
AES antibody can be used for the detection of AES by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
AES Antibody: Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis. It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.
Background References:
  • Martin SS and Vuori K. Regulation of Bcl-2 proteins during anoikis and amorphosis. Biochim Biophys Acta. 2004; 1692:145-57.
  • Miyasaka H, Choudhury BK, Hou WE, et al. Molecular cloning and expression of mouse and human cDNA encoding AES and ESG proteins with strong similarity to Drosophila enhancer of split groucho protein. Eur. J. Biochem. 1993; 216:343-52.
  • Chen G and Courey AJ. Groucho/TLE family proteins and transcriptional repression. Gene 2000; 249:1-16.
  • Jan Y, Matter M, Pai J-t, et al. A mitochondrial protein, Bit1, mediates apoptosis regulated by integrins and groucho/TLE corepressors. Cell 2004; 116:751-762.
Buffer:
AES Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
AES antibody was raised against a 16 amino acid synthetic peptide from near the amino-terminus of human AES.

The immunogen is located within the first 50 amino acids of AES.
NCBI Gene ID #:
166
NCBI Official Name:
amino-terminal enhancer of split
NCBI Official Symbol:
AES
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_945320
Protein GI Number:
39812019
Purification:
AES Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis,Neuroscience
Swissprot #:
Q08117
User NOte:
Optimal dilutions for each application to be determined by the researcher.

Related Products

Product NameProduct CodeSupplier 
AES Peptide PSI-3609PProSci Summary Details