Beclin-1 Antibody

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ProSci
Product Code: PSI-3613
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3613-0.02mg0.02mg£150.00
Quantity:
PSI-3613-0.1mg0.1mg£449.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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<strong>Figure 1 WB Validation in Human Cell Lines </strong><br> Loading: 15 μg of lysate Antibodies: Beclin-1 3613 , 1 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong> Figure 2 WB Validation in Mouse Cell Lines</strong><br> Loading: 15 μg of lysate Antibodies: Beclin-1 3613 , 1 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 3 WB Validation in Rat Cell Lines</strong><br> Loading: 15 μg of lysate Antibodies: Beclin-1 3613, 1 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 4 Immunofluorescence Validation of Beclin-1 in HELA Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed Hela cells labeling Beclin-1 with 3613 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).
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<strong>Figure 5 Immunofluorescence Validation of Beclin-1 in Mouse Brain Tissue</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain cells labeling Beclin-1 with 3613 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).
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<strong>Figure 6 Immunohistochemistry Validation of Beclin-1 in Human Brain Tissue</strong><br> Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-Beclin-1 antibody (3613) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
7 / 13
<strong>Figure 7 Immunohistochemistry Validation of Beclin-1 in Mouse Kidney Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Beclin-1 antibody (3613) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
8 / 13
<strong>Figure 8 Immunohistochemistry Validation of Beclin-1 in Rat Liver Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Beclin-1 antibody (3613) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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<strong>Figure 9 Immunohistochemistry Validation of Beclin-1 in Rat Spleen Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-Beclin-1 antibody (3613) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
10 / 13
<strong>Figure 10 Knockdown of Beclin-1 in Human T47D Cells (Thomas et al., 2011) </strong><br> T47D cells were transfected with scrambled or Beclin-1 siRNA. The following day, these cells were treated with vehicle, C, 0.5 mM VPA, 10 lM 4OH-tamoxifen, T, or 0.5 mM VPA and 10 lM 4OH-tamoxifen, VT, for 48 h and viability was assayed by dye exclusion.
11 / 13
<strong>Figure 11 Immunofluorescent Validation of Beclin-1 in Human Cortical Neurons and Rat Brain (Wang et al., 2017) </strong><br> Cell specificity of Beclin 1 expression. Cortical neurons and glia co-cultures (upper panels) and cortex slices (lower panels) were immunostained with anti-Beclin 1 (red) and NeuN (neuronal biomarker, green) or GFAP (astrocyte biomarker, green). Neurons and astrocyte in cultured cells were indicated by arrows and arrowhead, respectively. Areas in white boxes were enlarged.
12 / 13
<strong>Figure 12 Immunohistochemistry Validation of Beclin-1 in Human HCC Cells (Qiu et al., 2014) </strong><br> Beclin-1 exhibited cytoplasmic staining in adjacent non-tumor (ANT) tissues (A), bordeling site between HCC and ANT (B: left, ANT; right: HCC), and HCC (C), respectively. Beclin-1 expression was stronger in ANT than in HCC. Magnification x 400.
13 / 13
<strong>Figure 13 Regulated Expression of Beclin-1 in Human T47D Cells (Thomas et al., 2011) </strong><br> Treatment with tamoxifen and an HDAC inhibitor alters the balance of autophagy and apoptosis inhibitors and drivers. T47D cells were treated with vehicle, C, 0.5 mM VPA, V, 10 lM 4OHtamoxifen, T, or 0.5 mM VPA and 10 lM 4OH-tamoxifen, VT, for 48 h and Beclin-1 protein and RNA
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<strong>Figure 1 WB Validation in Human Cell Lines </strong><br> Loading: 15 μg of lysate Antibodies: Beclin-1 3613 , 1 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong> Figure 2 WB Validation in Mouse Cell Lines</strong><br> Loading: 15 μg of lysate Antibodies: Beclin-1 3613 , 1 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 WB Validation in Rat Cell Lines</strong><br> Loading: 15 μg of lysate Antibodies: Beclin-1 3613, 1 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Immunofluorescence Validation of Beclin-1 in HELA Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed Hela cells labeling Beclin-1 with 3613 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).
<strong>Figure 5 Immunofluorescence Validation of Beclin-1 in Mouse Brain Tissue</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain cells labeling Beclin-1 with 3613 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).
<strong>Figure 6 Immunohistochemistry Validation of Beclin-1 in Human Brain Tissue</strong><br> Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-Beclin-1 antibody (3613) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 7 Immunohistochemistry Validation of Beclin-1 in Mouse Kidney Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Beclin-1 antibody (3613) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 8 Immunohistochemistry Validation of Beclin-1 in Rat Liver Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Beclin-1 antibody (3613) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 9 Immunohistochemistry Validation of Beclin-1 in Rat Spleen Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-Beclin-1 antibody (3613) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 10 Knockdown of Beclin-1 in Human T47D Cells (Thomas et al., 2011) </strong><br> T47D cells were transfected with scrambled or Beclin-1 siRNA. The following day, these cells were treated with vehicle, C, 0.5 mM VPA, 10 lM 4OH-tamoxifen, T, or 0.5 mM VPA and 10 lM 4OH-tamoxifen, VT, for 48 h and viability was assayed by dye exclusion.
<strong>Figure 11 Immunofluorescent Validation of Beclin-1 in Human Cortical Neurons and Rat Brain (Wang et al., 2017) </strong><br> Cell specificity of Beclin 1 expression. Cortical neurons and glia co-cultures (upper panels) and cortex slices (lower panels) were immunostained with anti-Beclin 1 (red) and NeuN (neuronal biomarker, green) or GFAP (astrocyte biomarker, green). Neurons and astrocyte in cultured cells were indicated by arrows and arrowhead, respectively. Areas in white boxes were enlarged.
<strong>Figure 12 Immunohistochemistry Validation of Beclin-1 in Human HCC Cells (Qiu et al., 2014) </strong><br> Beclin-1 exhibited cytoplasmic staining in adjacent non-tumor (ANT) tissues (A), bordeling site between HCC and ANT (B: left, ANT; right: HCC), and HCC (C), respectively. Beclin-1 expression was stronger in ANT than in HCC. Magnification x 400.
<strong>Figure 13 Regulated Expression of Beclin-1 in Human T47D Cells (Thomas et al., 2011) </strong><br> Treatment with tamoxifen and an HDAC inhibitor alters the balance of autophagy and apoptosis inhibitors and drivers. T47D cells were treated with vehicle, C, 0.5 mM VPA, V, 10 lM 4OHtamoxifen, T, or 0.5 mM VPA and 10 lM 4OH-tamoxifen, VT, for 48 h and Beclin-1 protein and RNA

Documents

Further Information

Additional Names:
Beclin-1 Antibody: ATG6, VPS30, beclin1, GT197, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein
Application Note:
WB: 1 μg/mL; IHC: 2 μg/mL; IF: 20 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human, mouse, and rat samples; Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
Background:
Beclin-1 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. Beclin-1, a coiled-coil Bcl-2-interacting protein homologous to the yeast autophagy gene apg6, is a mammalian autophagy gene that can inhibit tumorigenesis and is expressed at reduced levels in human breast carcinoma, suggesting that defects in autophagy proteins may contribute to the development or progression of tumors. Bcl-2 can bind to Beclin-1 and inhibit Beclin-1-dependent autophagy in yeast and mammalian cells, suggesting that Bcl-2 functions as an anti-autophagy protein as well as an anti-apoptotic protein, which helps maintain autophagy at levels that are more compatible with cell survival rather than cell death.
Background References:
  • Gozuacik et al. Oncogene. 2004; 23:2891-906.
  • Kisen et al. Carcinogenesis 1993; 14:2501-5.
  • Liang et al. J. Virol. 1998; 72:8586-96.
  • Kametaka et al. J. Biol. Chem. 1998; 273:22284-91.
Buffer:
Beclin-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (94%), Pig: (88%)
Immunogen:
Anti-Beclin-1 antibody (3613) was raised against a peptide corresponding to 17 amino acids near the center of human Beclin-1.

The immunogen is located between 40-90 amino acids of Beclin-1.
ISOFORMS:
Human Beclin-1 has one isoform (450aa, 52kD). Mouse Beclin-1 has one isoform (448aa, 52kD) and Rat Beclin-1 also has one isoform (448aa, 52kD). 3613 can detect human, mouse and rat.
NCBI Gene ID #:
8678
NCBI Official Name:
beclin 1, autophagy related
NCBI Official Symbol:
BECN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 52 kD

Observed: 60 kD
Protein Accession #:
AAH10276
Protein GI Number:
16307457
Purification:
Beclin-1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis,Autophagy,Cancer
Swissprot #:
Q14457
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Antibody validated: Western Blot in human samples; Immunohistochemistry in human, mouse, and rat samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.

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