ProSci

LAMP-2 Antibody

Product Code:
 
PSI-3627
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
1 / 3
Western blot analysis of LAMP-2 in HepG2 cell lysate with LAMP-2 antibody at (A) 1 and (B) 2 μg/mL.
2 / 3
Immunocytochemistry of LAMP-2 in HepG2 cells with LAMP-2 antibody at 10 μg/mL.
3 / 3
Immunohistochemistry of LAMP-2 in mouse liver tissue with LAMP-2 Antibody at 5 μg/mL.
❮❯

Western blot analysis of LAMP-2 in HepG2 cell lysate with LAMP-2 antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of LAMP-2 in HepG2 cells with LAMP-2 antibody at 10 μg/mL.
Immunohistochemistry of LAMP-2 in mouse liver tissue with LAMP-2 Antibody at 5 μg/mL.

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CodeSizePrice
PSI-3627-0.02mg0.02mg£150.00
Quantity:
PSI-3627-0.1mg0.1mg£449.00
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Further Information

Additional Names:
LAMP-2 Antibody: Mac3, LGP-B, CD107b, Lamp-2, Lamp II, Lamp-2a, Lamp-2b, Lamp-2c, Lysosome-associated membrane glycoprotein 2, CD107 antigen-like family member B, LAMP-2
Application Note:
LAMP-2 antibody can be used for the detection of LAMP-2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL and immunohistochemistry starting at 5 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Background:
LAMP-2 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components and is negatively regulated by TOR (Target of rapamycin). LAMP-2, a highly glycosylated protein associated with the lysosome, has recently been shown to be important in autophagy as mice deficient in this protein failed to convert autophagic vacuoles into vacuoles leading to impaired degradation of long-lived proteins. This correlates with the finding that human LAMP-2 deficiency causing Danon's disease is associated with the accumulation of autophagic material in striated myocytes. LAMP-2 exists in multiple isoforms.
Background References:
  • Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906.
  • Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis1993; 14:2501-5.
  • Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol.2000; 150:1507-13.
  • Granger BL, Green SA, Gabel CA, et al. Characterization and cloning of the lgp110, a lysosomal glycoprotein from mouse and rat cells. J. Biol. Chem.1990; 265:12036-43.
Buffer:
LAMP-2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (75%), Rat: (75%), Bovine: (75%)
Immunogen:
LAMP-2 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human LAMP-2.

The immunogen is located within the last 50 amino acids of LAMP-2.
NCBI Gene ID #:
16784
NCBI Official Name:
lysosomal-associated membrane protein 2
NCBI Official Symbol:
Lamp2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_054701
Protein GI Number:
31543108
Purification:
LAMP-2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis,Autophagy
SPECIFICITY:
LAMP-2 antibody cross reacts with LAMP2B and LAMP2C, but not LAMP2A.
Swissprot #:
P17047
User NOte:
Optimal dilutions for each application to be determined by the researcher.