ProSci

TLR3 Antibody

Product Code:
 
PSI-3643
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
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Western blot analysis of TLR3 in Daudi cell lysate with TLR3 antibody at (A) 1 and (B) 2 μg/mL.
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Immunocytochemistry of TLR3 in EL4 cells with TLR3 antibody at 1 μg/mL.
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Immunofluorescence of TLR3 in El4 cells with TLR3 antibody at 10 μg/mL.
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Immunohistochemistry of TLR3 in human spleen tissue with TLR3 antibody at 5 μg/ml.
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Immunohistochemistry of TLR3 in mouse spleen tissue with TLR3 antibody at 2 μg/ml.
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Immunofluorescence of TLR3 in mouse spleen tissue with TLR3 antibody at 20 μg/ml.<br><br>Green: TLR3 Antibody (3643) <br> Blue: DAPI staining
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Immunofluorescence of TLR3 in human spleen tissue with TLR3 antibody at 20 μg/ml.<br><br>Green: TLR3 Antibody (3643) <br> Blue: DAPI staining
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Western blot analysis of TLR3 expression in mouse spleen tissue lysate with TLR3 antibody at (A) 0.5 and (B) 1 μg/ml.
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Western blot analysis of TLR3 in Daudi cell lysate with TLR3 antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of TLR3 in EL4 cells with TLR3 antibody at 1 μg/mL.
Immunofluorescence of TLR3 in El4 cells with TLR3 antibody at 10 μg/mL.
Immunohistochemistry of TLR3 in human spleen tissue with TLR3 antibody at 5 μg/ml.
Immunohistochemistry of TLR3 in mouse spleen tissue with TLR3 antibody at 2 μg/ml.
Immunofluorescence of TLR3 in mouse spleen tissue with TLR3 antibody at 20 μg/ml.<br><br>Green: TLR3 Antibody (3643) <br> Blue: DAPI staining
Immunofluorescence of TLR3 in human spleen tissue with TLR3 antibody at 20 μg/ml.<br><br>Green: TLR3 Antibody (3643) <br> Blue: DAPI staining
Western blot analysis of TLR3 expression in mouse spleen tissue lysate with TLR3 antibody at (A) 0.5 and (B) 1 μg/ml.

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PSI-3643-0.02mg0.02mg£150.00
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PSI-3643-0.1mg0.1mg£449.00
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Further Information

Additional Names:
TLR3 Antibody: CD283, IIAE2, Toll-like receptor 3
Application Note:
TLR3 antibody can be used for detection of TLR3 by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL and immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.

Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human and mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
Background:
TLR3 Antibody: Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The TLRs act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors so the organism can respond to potential infection. TLR3 is known to recognize viral double-stranded (ds) RNA, a molecular pattern associated with viral infection. Recently it has been shown to recognize viruses such as Influenza A and West Nile Virus and can mediate entry of at least West Nile Virus.
Background References:
  • Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76.
  • Janeway CA Jr. and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002; 20:197-216.
  • McGettrick AF and O'Neill LAJ. The expanding family of MyD88-like adaptors in Toll-like receptor signal transduction. Mol Imm. 2004; 41:577-82.
  • Alexopoulou L, Holt AC, Medzhitov R, et al. Recognition of double-stranded RNA and activation of NF-κB by Toll-like receptor 3. Nature 2001; 413:732-8.
Buffer:
TLR3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (93%)
Immunogen:
TLR3 antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human TLR3.

The immunogen is located within amino acids 780 - 830 of TLR3.
NCBI Gene ID #:
7098
NCBI Official Name:
toll-like receptor 3
NCBI Official Symbol:
TLR3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 99 kDa

Observed: 105 kDa
Protein Accession #:
O15455
Protein GI Number:
20140422
Purification:
TLR3 Antibody is affinity chromatography purified via peptide column.
Research Area:
Innate Immunity
Swissprot #:
O15455
User NOte:
Optimal dilutions for each application to be determined by the researcher.