ProSci

BAP31 Antibody

Product Code:
 
PSI-3675
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
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<strong>Figure 1 Western Blot Validation in Rat Lung Tissue Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: BAP31, 3675 (A: 0.5 μg/mL, B: 1 μg/mL and C: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: BAP31, 3675 (0.5 μg/mL), BAP31, 64-237 (2 μg/mL), and beta-actin 3779 (1.5 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 3 Western Blot Validation in Human, Mouse (NIH/3T3) and Rat (YB2/0) Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: BAP31, 3675 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 4 Immunohistochemistry Validation of BAP31 in Rat Lung Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-BAP31 antibody (3675) at 10 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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<strong>Figure 1 Western Blot Validation in Rat Lung Tissue Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: BAP31, 3675 (A: 0.5 μg/mL, B: 1 μg/mL and C: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: BAP31, 3675 (0.5 μg/mL), BAP31, 64-237 (2 μg/mL), and beta-actin 3779 (1.5 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation in Human, Mouse (NIH/3T3) and Rat (YB2/0) Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: BAP31, 3675 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Immunohistochemistry Validation of BAP31 in Rat Lung Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-BAP31 antibody (3675) at 10 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

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PSI-3675-0.02mg0.02mg£150.00
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Further Information

Additional Names:
BAP31 Antibody: CDM, DDCH, BAP31, 6C6-AG, DXS1357E, B-cell receptor-associated protein 31, 6C6-AG tumor-associated antigen, BCR-associated protein 31
Application Note:
WB: 0.5-2 μg/mL; IHC: 10 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in rat samples. All other applications and species not yet tested.
Background:
BAP31 Antibody: Bap31 and the related protein Bap29 are endoplasmic reticulum (ER) and ER-vesicle membrane proteins and members of the B-cell receptor-associated protein family. These two proteins are highly homologous and can form homo- and heterodimers. Bap31 is thought to be involved in the intracellular trafficking of several molecules such as MHC Class I molecules and CD11b/CD18. Bap31 may also play a role in the initiation of ER stress-induced apoptosis through its association with caspase-8 via a death effector domain in its cytoplasmic tail, possibly through the promotion of membrane fragmentation and the release of cytochrome c from mitochondria. Bap31 itself contains two caspase cleavage sites and is cleaved during apoptosis. The p20 fragment of Bap31, when expressed ectopically, is also a potent inducer cell death.
Background References:
  • Ng et al. J. Cell Biol.1997; 139:327-38.
  • Kim et al. EMBO J.1994; 13:3793-800.
  • Zen et al. J. Biol. Chem.2004; 279:44924-30.
Buffer:
BAP31 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Anti-BAP31 antibody (3675) was raised against a peptide corresponding to 16 amino acids near the center of human BAP31.

The immunogen is located within amino acids 130 - 180 of BAP31.
ISOFORMS:
Human BAP31 has 2 isoforms, including isoform 1 (246aa, 28kD) and isoform 2 (313aa, 35kD). Mouse BAP31 has one isoform (245aa, 28kD) and Rat BAP31 also has one isoform (245aa, 28kD). 3675 can detect human, mouse and rat.
NCBI Gene ID #:
10134
NCBI Official Name:
B-cell receptor-associated protein 31
NCBI Official Symbol:
BCAP31
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 28 or 34kD

Observed: 28 kD
Protein Accession #:
NP_005736
Protein GI Number:
32171186
Purification:
BAP31 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
Swissprot #:
P51572
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:

Independent Antibody Validation in Cell lines (Figure 2) shows similar BAP31 expression profile in human cell lines detected by two independent anti-BAP31 antibodies that recognize different epitopes, 3675 against central domain and 64-237, against recombinant protein.  BAP31 proteins are detected in all tested cell lines at different expression levels by the two independent antibodies.