ASK1 Antibody
| Code | Size | Price |
|---|
| PSI-3679-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-3679-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Documents
Further Information
Additional Names:
ASK1 Antibody: ASK1, MEKK5, MAPKKK5, ASK1, Mitogen-activated protein kinase kinase kinase 5, Apoptosis signal-regulating kinase 1, ASK-1
Application Note:
ASK1 antibody can be used for the detection of ASK1 by Western blot at 1 - 2 μg/mL. 3
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Background:
ASK1 Antibody: Mitogen-activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5. ASK1 activated two different subgroups of MAPKK, MKK4 and MKK6, which in turn activated c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death, and a catalytically inactive form of ASK1 inhibited TNF-α-induced apoptosis. ASK1 is expressed in variety of tissues and cell lines.
Background References:
- Ichijo H, Nishida E, Irie K, et al. Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways. Science1997; 275:90-4.
- Wang XS, Diener K, Jannuzzi D, et al. Molecular cloning and characterization of a novel protein kinase with a catalytic domain homologous to mitogen-activated protein kinase kinase kinase. J. Biol. Chem.1996; 271:31607-11.
Buffer:
ASK1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
ASK1 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human ASK1.
The immunogen is located within amino acids 390 - 440 of ASK1.
The immunogen is located within amino acids 390 - 440 of ASK1.
NCBI Gene ID #:
4217
NCBI Official Name:
mitogen-activated protein kinase kinase kinase 5
NCBI Official Symbol:
MAP3K5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
Q99683
Protein GI Number:
6685617
Purification:
ASK1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis
Swissprot #:
Q99683
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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