TLR9 Antibody

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ProSci
Product Code: PSI-3739
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3739-0.02mg0.02mg£150.00
Quantity:
PSI-3739-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of TLR9 in Jurkat cell lysate with TLR9 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide
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Immunocytochemistry of TLR9 in Jurkat cells with TLR9 antibody at 2 μg/mL.
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Immunofluorescence of TLR9 in Mouse Spleen tissue with TLR9 antibody at 10 μg/mL.
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Western blot analysis of TLR9 in Jurkat cell lysate with TLR9 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide
Immunocytochemistry of TLR9 in Jurkat cells with TLR9 antibody at 2 μg/mL.
Immunofluorescence of TLR9 in Mouse Spleen tissue with TLR9 antibody at 10 μg/mL.

Documents

Further Information

Additional Names:
TLR9 Antibody: CD289, Toll-like receptor 9
Application Note:
TLR9 antibody can be used for detection of TLR9 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
TLR9 Antibody: Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. TLR9 forms a subfamily along with TLR7 and TLR8 that recognize viral RNA and CpG DNA sequences and are localized in intracellular acidic compartments such as the phagolysosome. Unlike other TLRs which act through adaptor molecules such as TOLLIP, TIRAP, TRIF, and MyD88 to activate various kinases and transcription factors to respond to potential infection, TLR9 is strictly dependent on MyD88.
Background References:
  • Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol.2003; 21:335-76.
  • Janeway CA Jr. and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol.2002; 20:197-216.
  • Wagner H. The immunobiology of the TLR9 subfamily. Trends Immunol.2004; 381-6
  • Nishiya T and DeFranco AL. Ligand-regulated chimeric receptor approach reveals distinctive subcellular localization and signaling properties of the Toll-like receptors. J. Biol. Chem.2004; 279:19008-17.
Buffer:
TLR9 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
TLR9 antibody was raised against a peptide corresponding to 15 amino acids near the center of human TLR9.

The immunogen is located within amino acids 840 - 890 of TLR9.
NCBI Gene ID #:
54106
NCBI Official Name:
toll-like receptor 9
NCBI Official Symbol:
TLR9
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 104, 111 kDa

Observed: 97 kDa
Protein Accession #:
AAH32713
Protein GI Number:
21595773
Purification:
TLR9 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction,Innate Immunity
SPECIFICITY:
At least two isoforms of TLR9 are known to exist; this antibody will detect both isoforms. TLR9 antibody is predicted to not cross-react with other TLR proteins.
Swissprot #:
Q9NR96
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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