TOLLIP Antibody

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ProSci
Product Code: PSI-3743
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3743-0.02mg0.02mg£150.00
Quantity:
PSI-3743-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of TOLLIP in rat brain cell lysate with TOLLIP antibody at (A) 0.5, (B) 1 and (C) 2μg/mL.
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Immunocytochemistry of TOLLIP in THP-1 cells with TOLLIP antibody at 2 μg/mL.
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Western blot analysis of TOLLIP in rat brain cell lysate with TOLLIP antibody at (A) 0.5, (B) 1 and (C) 2μg/mL.
Immunocytochemistry of TOLLIP in THP-1 cells with TOLLIP antibody at 2 μg/mL.

Documents

Further Information

Additional Names:
TOLLIP Antibody: IL-1RAcPIP, Toll-interacting protein
Application Note:
TOLLIP antibody can be used for the detection of TOLLIP by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL.

Antibody validated: Western Blot in rat samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Background:
TOLLIP Antibody: Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The TLRs act through adaptor molecules to activate various kinases and transcription factors so the organism can respond to potential infection. These adaptor molecules include TOLLIP, MyD88, and TRIF. TOLLIP associates directly with TLR2 and TLR 4, acting as an inhibitor to TLR activation. This negative regulation of TLR signaling may serve to limit the production of proinflammatory mediators during infection and inflammation.
Background References:
  • Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol.2003; 21:335-76.
  • Janeway CA Jr. and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol.2002; 20:197-216.
  • McGettrick AF and O'Neill LAJ. The expanding family of MyD88-like adaptors in Toll-like receptor signal transduction. Mol. Imm.2004; 41:577-82.
  • Zhang G and Ghosh S. Negative regulation of Toll-like receptor-mediated signaling by Tollip. J. Biol. Chem.2002; 277:7059-65.
Buffer:
TOLLIP Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Chicken: (100%)
Immunogen:
TOLLIP antibody was raised against a 16 amino acid synthetic peptide from near the center of human TOLLIP.

The immunogen is located within amino acids 70 - 120 of TOLLIP.
NCBI Gene ID #:
54472
NCBI Official Name:
toll interacting protein
NCBI Official Symbol:
TOLLIP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
AAH18272
Protein GI Number:
17390641
Purification:
TOLLIP Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
Swissprot #:
Q9H0E2
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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