TWEAK Antibody
Code | Size | Price |
---|
PSI-3791-0.02mg | 0.02mg | £150.00 |
Quantity:
PSI-3791-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Images
Documents
Further Information
Additional Names:
TWEAK Antibody: APO3L, DR3LG, TWEAK, APO3L, UNQ181/PRO207, Tumor necrosis factor ligand superfamily member 12, APO3 ligand
Application Note:
WB: 0.5-4 μg/mL; IHC: 5-10 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human and mouse samples; Immunofluorescence in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human and mouse samples; Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
TWEAK Antibody: TNF-related weak inducer of apoptosis (TWEAK) is a member of the tumor necrosis factor superfamily (TNFSF) of structurally related cytokines. Like most other members of this family, TWEAK is a cell surface-associated type II transmembrane protein although a smaller, biologically active form can also be generated by cleavage near the cell membrane. TWEAK has multiple biological activities, including stimulation of cell growth and angiogenesis, induction of inflammatory cytokines, in addition to stimulation of apoptosis. The TWEAK signal transduction pathway has not been well established but it appears to signal via TweakR/Fn14 in a manner similar to that described for other TNFSF members that bind receptors lacking death domains.
Background References:
- Wiley and Winkles. Cyto. And Growth Factor Rev. 2003; 14:241-9.
- Chicheportiche et al. J. Biol. Chem. 1997; 272:32401-10.
- Lynch et al. J. Biol. Chem. 1999; 274:8455-9.
- Chicheportiche et al. Arthr. Res. 2002; 4:126-33. 5.
Buffer:
TWEAK Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Anti-TWEAK antibody (3791) was raised against recombinant human TWEAK protein.
ISOFORMS:
Human TWEAK has 2 isoforms, including isoform 1 (249aa, 27kD) and isoform 2 (330aa, 36kD). Mouse TWEAK has one isoform (249aa, 27kD) and Rat TWEAK also has one isoform (249aa, 27kD).
NCBI Gene ID #:
8742
NCBI Official Name:
tumor necrosis factor (ligand) superfamily, member 12
NCBI Official Symbol:
TNFSF12
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 27 and 36 kD
Observed: 27 and 36 kD
Observed: 27 and 36 kD
Protein Accession #:
NP_003800
Protein GI Number:
4507597
Purification:
TWEAK Antibody is Protein A-purified.
Research Area:
Apoptosis,Cancer
Swissprot #:
O43508
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Recombinant Protein Test (Figure 2): Anti-TWEAK antibodies (3791) detected human TWEAK recombinant protein at different concentrations.