ATF6 Antibody

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ProSci
Product Code: PSI-3683
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3683-0.02mg0.02mg£150.00
Quantity:
PSI-3683-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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<strong>Figure 1 KO Validation of ATF6 </strong><br> Loading: 15 μg of human HeLa cell lysate Antibodies ATF6 3683, 0.5 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 2 WB Validation in Human Cell Lines </strong><br> Loading: 15 μg of human cell lysate Antibodies ATF6 3683, 1 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. conjugate at 1:10000 dilution.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. conjugate at 1:10000 dilution.
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<strong>Figure 3 WB Validation in Mouse Tissue </strong><br> Loading: 15 μg of mouse tissue lysate Antibodies: ATF6 3683, 1 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 4 WB Validation in Rat Heart </strong><br> Loading: 15 μg of rat kidney lysate Antibodies: ATF6 3683, 1 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 5 Immunofluorescence Validation of ATF6 In A549 Cells </strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed A549 cells labeling ATF6 with 3683 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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<strong>Figure 6 Immunofluorescence Validation of ATF6 in Human Testis Cells </strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human testis labeling ATF6 with 3683 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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<strong>Figure 7 Immunohistochemistry Validation of ATF6 in Mouse Brain Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ATF6 antibody (3683) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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<strong>Figure 8 Immunohistochemistry Validation of ATF6 in Rat Brain Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded Rat brain tissue using anti-ATF6 antibody (3683) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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<strong>Figure 1 KO Validation of ATF6 </strong><br> Loading: 15 μg of human HeLa cell lysate Antibodies ATF6 3683, 0.5 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 WB Validation in Human Cell Lines </strong><br> Loading: 15 μg of human cell lysate Antibodies ATF6 3683, 1 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. conjugate at 1:10000 dilution.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. conjugate at 1:10000 dilution.
<strong>Figure 3 WB Validation in Mouse Tissue </strong><br> Loading: 15 μg of mouse tissue lysate Antibodies: ATF6 3683, 1 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 WB Validation in Rat Heart </strong><br> Loading: 15 μg of rat kidney lysate Antibodies: ATF6 3683, 1 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 5 Immunofluorescence Validation of ATF6 In A549 Cells </strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed A549 cells labeling ATF6 with 3683 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 6 Immunofluorescence Validation of ATF6 in Human Testis Cells </strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human testis labeling ATF6 with 3683 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 7 Immunohistochemistry Validation of ATF6 in Mouse Brain Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ATF6 antibody (3683) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 8 Immunohistochemistry Validation of ATF6 in Rat Brain Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded Rat brain tissue using anti-ATF6 antibody (3683) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Documents

Further Information

Additional Names:
ATF6 Antibody: ATF6A, Cyclic AMP-dependent transcription factor ATF-6 alpha, Activating transcription factor 6 alpha, cAMP-dependent transcription factor ATF-6 alpha
Application Note:
WB: 0.5-1 μg/mL; IHC: 5 μg/mL; IF: 20 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in mouse and rat samples; Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
ATF6 Antibody: Disruptions of protein folding and maturation in the endoplasmic reticulum (ER) result in the activation of the unfolded protein response (UPR), an integrated cellular signaling pathway that transmits information from the ER lumen to the cytoplasm and nucleus. Activating transcription factor 6 (ATF6) as well as the ER-transmembrane protein kinases IRE1p and PERK are the major transducers of the UPR. ATF6 is an ER transmembrane protein that is normally bound to the ER chaperone GRP78, but upon ER stress is released from GRP78 and proteolytically cleaved to yield a cytosolic fragment which then migrates to the nucleus, and together with the transcription factor XBP-1, activates transcription of UPR-responsive genes. ATF6 has two isoforms (ATF6α and ATF6β); only ATF6α is recognized by this antibody.
Background References:
  • Liu CY and Kaufman RJ. J. Cell Sci. 2003; 1861-2.
  • Haze K, et al. Mol. Cell. Biol. 1999; 10:3787-99.
  • Little E, et al. Crit. Rev. Eukaryot. Gene Expr. 1994; 4:1-18
  • Yoshida H, et al. Cell 2001; 107:881-91.
Buffer:
ATF6 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
ATF6 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human ATF6.

The immunogen is located within amino acids 30 - 80 of ATF6.
ISOFORMS:
Human ATF6 has one isoform (670aa, 74.6kD). Mouse ATF6 has one isoform (656aa, 72.7kD) and rat ATF6 also has one isoform (656aa, 72.7kD). 3683 can detect human, mouse and rat.
NCBI Gene ID #:
22926
NCBI Official Name:
activating transcription factor 6
NCBI Official Symbol:
ATF6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_031374
Protein GI Number:
56786157
Purification:
ATF6 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction,Chemokines & Cytokines
Swissprot #:
P18850
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
KO Validation (Figure 1): Anti-ATF6 antibodies (3683) specificity was further verified by ATF6 specific knockout. ATF6 signal was not detected in ATF6 knockout HeLa cells as compared to that in control wild type cells.

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