XBP-1 Antibody

Contact us
ProSci
Product Code: PSI-3685
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3685-0.02mg0.02mg£150.00
Quantity:
PSI-3685-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

1 / 2
Western blot analysis of XBP-1 in PC-3 cell lysate with XBP-1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
2 / 2
Immunocytochemistry of XBP-1 in HepG2 cells with XBP-1 antibody at 2 μg/mL.
❮❯

Western blot analysis of XBP-1 in PC-3 cell lysate with XBP-1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Immunocytochemistry of XBP-1 in HepG2 cells with XBP-1 antibody at 2 μg/mL.

Documents

Further Information

Additional Names:
XBP-1 Antibody: XBP2, TREB5, XBP-1, XBP2, X-box-binding protein 1, Tax-responsive element-binding protein 5
Application Note:
XBP-1 antibody can be used for the detection of XBP-1 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL.

Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Background:
XBP-1 Antibody: X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of malfolded proteins in the endoplasmic reticulum (ER). Upon sensing unfolded proteins, an ER transmembrane endonuclease and kinase termed IRE1p is activated and excises an intron from XBP-1 mRNA. The spliced XBP-1 mRNA results in a 371 amino acid protein (XBP-1s) which is then translocated to the nucleus where it binds to the regulatory elements of downstream genes. Together with other UPR transcription factors such as ATF6, XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones glucose regulated protein (GRP) 78 and GRP94.
Background References:
  • Yoshida H, Matsui T, Yamamoto T, et al. XBP1 mRNA is induced by ATF6 and spliced by IRE1p in response to ER stress to produce a highly active transcription factor. Cell 2001; 107:881-91.
  • Calfon M, Zeng H, Urano F, et al. IRE1 couples endoplasmic reticulum load to secretory capacity by processing the XBP-1 mRNA. Nature 2002; 415:92-6.
  • Haze K, Yoshida H, Yanagi H, et al. Mammalian transcription factor ATF6 is synthesized as a transmembrane protein and activated by proteolysis in response to endoplasmic stress. Mol. Cell. Biol. 1999; 10:3787-99.
  • Little E, Ramakrishnan M, Roy B, et al. The glucose-regulated proteins (GRP78 and GRP94): functions, gene regulation, and applications. Crit. Rev. Eukaryot. Gene Expr. 1994; 4:1-18.
Buffer:
XBP-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (83%)
Immunogen:
XBP-1 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human XBP-1.

The immunogen is located within the last 50 amino acids of XBP-1.
NCBI Gene ID #:
7494
NCBI Official Name:
X-box binding protein 1
NCBI Official Symbol:
XBP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
P17861
Protein GI Number:
60416406
Purification:
XBP-1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction,Chemokines & Cytokines
Swissprot #:
P17861
User NOte:
Optimal dilutions for each application to be determined by the researcher.

Related Products

Product NameProduct CodeSupplier 
XBP-1 PeptidePSI-3685PProSci Summary Details