IL-32 Antibody

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ProSci
Product Code: PSI-3749
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3749-0.02mg0.02mg£150.00
Quantity:
PSI-3749-0.1mg0.1mg£449.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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<strong>Figure 1 Western Blot Validation with Recombinant Protein</strong><br> Loading: 30 ng of human IL-32 recombinant protein per lane. Antibodies: IL-32, 3749 (Lane 1: 0.5 μg/mL, Lane 2: 1 μg/mL, Lane 3: 2 μg/mL and Lane 4: 4 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 17kD.
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<strong>Figure 2 Western Blot Validation in Human Spleen Lysate, showing two isoforms of IL-32</strong><br> Loading: 15 μg of lysates per lane. Antibodies: IL-32, 3749 (lane 1: 2 μg/mL and lane 2: 4 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 3 Immunohistochemistry Validation of IL-32 in Human Spleen Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL-32 antibody (3749) at 10 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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<strong>Figure 4 Immunofluorescence Validation of IL-32 in Human Spleen Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen cells labeling IL-32 with 3749 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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<strong>Figure 5 Immunohistochemistry Validation of IL-32 in Mouse Spleen Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-IL-32 antibody (3749) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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<strong>Figure 1 Western Blot Validation with Recombinant Protein</strong><br> Loading: 30 ng of human IL-32 recombinant protein per lane. Antibodies: IL-32, 3749 (Lane 1: 0.5 μg/mL, Lane 2: 1 μg/mL, Lane 3: 2 μg/mL and Lane 4: 4 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 17kD.
<strong>Figure 2 Western Blot Validation in Human Spleen Lysate, showing two isoforms of IL-32</strong><br> Loading: 15 μg of lysates per lane. Antibodies: IL-32, 3749 (lane 1: 2 μg/mL and lane 2: 4 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Immunohistochemistry Validation of IL-32 in Human Spleen Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL-32 antibody (3749) at 10 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 4 Immunofluorescence Validation of IL-32 in Human Spleen Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen cells labeling IL-32 with 3749 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
<strong>Figure 5 Immunohistochemistry Validation of IL-32 in Mouse Spleen Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-IL-32 antibody (3749) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Documents

Further Information

Additional Names:
IL-32 Antibody: NK4, TAIF, TAIFa, TAIFb, TAIFc, TAIFd, IL-32beta, IL-32alpha, IL-32delta, IL-32gamma, NK4, Interleukin-32, Natural killer cells protein 4, IL-32
Application Note:
WB: 0.5-4 μg/mL; IHC: 2-10 μg/mL; IF: 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples; Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
IL-32 Antibody: Interleukin-32 (IL-32) was initially identified as a transcript (NK4) that is selectively expressed in lymphocytes and NK cells and whose expression is increased following activation by IL-2. It was later re-isolated from an IL-18-treated lung carcinoma cell line and re-named IL-32. IL-32 is unusual in that it does not share sequence homology with known cytokine families and is highly expressed in immune tissues, existing in at least four differentially spliced isoforms. Because treatment of human monocytic and mouse macrophage cells with IL-32 induces several proinflammatory cytokines such as TNF-α, IL-8 and MIP-2, and because it is also induced in human peripheral lymphocyte cells after mitogen stimulation and in epithelial cells by IFNγ, it has been suggested that IL-32 may play a role in autoimmune and inflammatory diseases such as rheumatoid arthritis.
Background References:
  • Dahl et al. J. Immunol. 1992; 148:597-603.
  • Kim et al. Immunity 2005; 22:131-42.
  • Cagnard et al. Eur. Cyto. Network 2005; 16:289-92.
Buffer:
IL-32 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Anti-IL-32 antibody (3749) was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human IL-32.

The immunogen is located within the last 50 amino acids of IL-32.
ISOFORMS:
Human IL-32 has 7 isoforms, including isoform 1 (234aa, 27kD), isoform 2 (188aa, 22kD), isoform 3 (178aa, 21kD), isoform 4 (131aa, 15kD), isoform 5 (168aa, 19kD), isoform 6 (179aa, 21kD) and isoform 7 (148aa, 17kD). 3749 can detect all human isoforms.
NCBI Gene ID #:
9235
NCBI Official Name:
interleukin 32
NCBI Official Symbol:
IL32
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 26kD

Observed: 26 kD
Protein Accession #:
AAH09401
Protein GI Number:
14424787
Purification:
IL-32 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction,Cancer
SPECIFICITY:
This antibody detects all isoforms of IL-32.
Swissprot #:
P24001
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:

Recombinant Protein Test (Figure 1): Anti-IL-32 antibodies (3749) detected human IL-32 recombinant protein at different concentrations.

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