BMI-1 Antibody

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ProSci
Product Code: PSI-3755
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3755-0.02mg0.02mg£150.00
Quantity:
PSI-3755-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of BMI-1 in K562 cell lysate with BMI-1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
2 / 3
Immunocytochemistry of BMI-1 in K562 cells with BMI-1 antibody at 10 μg/mL.
3 / 3
Immunofluorescence of BMI-1 in K562 cells with BMI-1 antibody at 20 μg/mL.
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Western blot analysis of BMI-1 in K562 cell lysate with BMI-1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Immunocytochemistry of BMI-1 in K562 cells with BMI-1 antibody at 10 μg/mL.
Immunofluorescence of BMI-1 in K562 cells with BMI-1 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
BMI-1 Antibody: PCGF4, RNF51, FLVI2/BMI1, PCGF4, Polycomb complex protein BMI-1, Polycomb group RING finger protein 4
Application Note:
BMI-1 antibody can be used for detection of BMI-1 by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
BMI-1 Antibody: The transcriptional repressor BMI-1 was first identified as a proto-oncogene frequently activated by Moloney murine leukemia proviral insertions in mice and cooperating with c-myc in the generation of mouse lymphomas. BMI-1 is involved in segment specification, cell growth and maintenance, transcriptional regulation, and chromatin modification. A major target of BMI-1 is the ink4a locus which encodes tumor suppressor proteins p16 and p19Arf, which are important in tumor progression and thought to be critical in cell proliferation and senescence. Recent studies have also shown that BMI-1 is required for the maintenance of adult normal and leukemic stem cells, suggesting that BMI-1 could an attractive therapeutic target for stem cell proliferation and renewal as well as for anti-cancer strategies.
Background References:
  • Alkema MJ, Wiegant J, Raap AK, et al. Characterization and chromosomal localization of the human proto-oncogene BMI-1. Hum. Mol. Genet. 1993; 2:1597-603.
  • Jacobs JJ, Kieboom K, Marino S, et al. The oncogene and polycomb-group gene bmi-1 regulates cell proliferation and senescence through the ink4a locus. Nature 1999; 397:164-8.
  • Lessard J and Sauvageau G. BMI-1 determines the proliferative capacity of normal and leukaemic stem cells. Nature 2003; 255-60.
Buffer:
BMI-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Chicken: (100%)
Immunogen:
BMI-1 antibody was raised against a peptide corresponding to 15 amino acids near the center of human BMI-1.

The immunogen is located within amino acids 140 - 190 of BMI-1.
NCBI Gene ID #:
648
NCBI Official Name:
BMI1 polycomb ring finger oncogene
NCBI Official Symbol:
BMI1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
P35226
Protein GI Number:
22258801
Purification:
BMI-1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction,Neuroscience
Swissprot #:
P35226
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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