DDX3 Antibody

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ProSci
Product Code: PSI-3757
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3757-0.02mg0.02mg£150.00
Quantity:
PSI-3757-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of DDX3 in HepG2 cell lysate with DDX3 antibody at (A) 0.5 and (B) 1 μg/mL.
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Immunocytochemistry of DDX3 in HepG2 cells with DDX3 antibody at 10 μg/mL.
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Immunofluorescence of DDX3 in HepG2 cells with DDX3 antibody at 20 μg/mL.
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Western blot analysis of DDX3 in HepG2 cell lysate with DDX3 antibody at (A) 0.5 and (B) 1 μg/mL.
Immunocytochemistry of DDX3 in HepG2 cells with DDX3 antibody at 10 μg/mL.
Immunofluorescence of DDX3 in HepG2 cells with DDX3 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
DDX3 Antibody: DBX, DDX3, HLP2, DDX14, DBX, ATP-dependent RNA helicase DDX3X, DEAD box protein 3, X-chromosomal
Application Note:
DDX3 antibody can be used for the detection of DDX3 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
DDX3 Antibody: DDX3 contains all of the motifs of the DEAD-box family of RNA helicases, including the Asp-Glu-Ala-Asp sequence that gives the protein family its name and distinguishes it from other RNA helicases. DDX3 is localized to the X chromosome and has a highly conserved functional homolog (DBY) on the Y chromosome. DDX3 is thought to be involved in RNA splicing, RNA transport, and translation initiation. It has also been shown to be involved in cell growth control and is deregulated in hepatitis virus-associated hepatocellular carcinoma. Recent experiments suppressing DDX3 expression blocked HIV-1 RNA export from the nucleus, suggesting that DDX3 functions as a shuttling protein that transports the HIV-1 protein Rev and its cofactor CRM1 from the nucleus to the cytoplasm.
Background References:
  • Linder P, Lasko PF, Ashburner M, et al. Birth of the D-E-A-D box. Nature 1989; 337:121-2.
  • Park SH, Lee SG, Kim Y, et al. Assignment of a human putative RNA helicase gene, DDX3, to human X chromosome bands p11.3-->p11.23. Cytogenet. Cell Genet. 1998; 81:178-9.
  • Lahn BT and Page DC. Functional coherence of the human Y chromosome. Science 1997; 278:675-80.
  • Abdelhaleem M. RNA helicases: regulators of differentiation. Clin. Biochem. 2005; 38:499-503.
Buffer:
DDX3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
DDX3 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human DDX3.

The immunogen is located within amino acids 110 - 160 of DDX3.
NCBI Gene ID #:
1654
NCBI Official Name:
DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked
NCBI Official Symbol:
DDX3X
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
AAC34298
Protein GI Number:
3523150
Purification:
DDX3 Antibody is affinity chromatography purified via peptide column.
Research Area:
Infectious Disease
SPECIFICITY:
DDX3 antibody will detect both DDX3 and DBY.
Swissprot #:
O00571
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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