LSD1 Antibody

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ProSci
Product Code: PSI-3763
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3763-0.02mg0.02mg£150.00
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PSI-3763-0.1mg0.1mg£449.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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<strong>Figure 1 Western Blot Validation in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: LSD1 3763 ( 1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: LSD1 3761 (0.2 μg/mL), LSD1 3763 (1 μg/mL), beta-actin 3779 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 3 Western Blot Validation in Mouse P815 Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: LSD1 3763, (A: 1 μg/mL, B: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 4 Immunohistochemistry Validation of LSD1 in Human Small Intestine Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-LSD1 antibody (3763) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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<strong>Figure 5 Immunofluorescence Validation of LSD1 in Human Small Intestine Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human small intestine cells labeling LSD1 with 3763 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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<strong>Figure 1 Western Blot Validation in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: LSD1 3763 ( 1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: LSD1 3761 (0.2 μg/mL), LSD1 3763 (1 μg/mL), beta-actin 3779 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation in Mouse P815 Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: LSD1 3763, (A: 1 μg/mL, B: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Immunohistochemistry Validation of LSD1 in Human Small Intestine Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-LSD1 antibody (3763) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 5 Immunofluorescence Validation of LSD1 in Human Small Intestine Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human small intestine cells labeling LSD1 with 3763 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Documents

Further Information

Additional Names:
LSD1 Antibody: AOF2, KDM1, LSD1, BHC110, AOF2, KIAA0601, Lysine-specific histone demethylase 1A, BRAF35-HDAC complex protein BHC110
Application Note:
WB: 0.2 - 2 μg/mL; IHC-P: 2 μg/mL; IF: 20 μg/mL

Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
LSD1 Antibody: Histone modifications mediate changes in gene expression by altering chromatin structure or by serving as a platform to recruit other proteins. LSD1 is a recently discovered amine oxidase that catalyzes the lysine-specific demethylation of histone proteins via an FAD-dependent oxidative reaction. Methylation on histone H3-K9 is thought to play an important role in heterochromatin formation, while methylation on arginine and some lysine residues (such as H3-K4) is associated with active transcription. LSD1 associates with various proteins, including HDAC1/2, CoREST, and BHC80, that act to regulate LSD1 activity in vivo, and in a histone H3-K4-specific methylase complex that is involved in transcriptional regulation. Experiments have shown that CoREST, a SANT domain-containing corepressor acts to enhance LSD1 activity, while BHC80, a PHD domain-containing protein, inhibits CoREST/LSD1 activity in vitro. LSD1-mediated histone demethylation thus may have significant effects on gene expression.
Background References:
  • Shi et al. Cell 2004; 119:941-53.
  • Kouzarides et al. Curr. Opin. Genet. Dev. 2002; 12:198-209.
  • Shi et al. Mol. Cell 2005; 19:857-64.
  • Nakamura et al. Mol. Cell 2002; 10:1119-28.
Buffer:
LSD1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Anti-LSD1 antibody (3763) was raised against a peptide corresponding to 16 amino acids near the center of human LSD1.

The immunogen is located within amino acids 140-190 of LSD1.
ISOFORMS:
Human LSD1 has 2 isoforms, including isoform 1 (852aa, 93kD) and isoform 2 (876aa, 95kD). Mouse LSD1 has one isoform (853aa, 93kD) and Rat LSD1 also has one isoform (872aa, 95kD). 3763 can detect all isoforms of human, mouse and rat.
NCBI Gene ID #:
23028
NCBI Official Name:
lysine demethylase 1A
NCBI Official Symbol:
KDM1A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 93kD

Observed: 110 kD
Protein Accession #:
NP_001009999
Protein GI Number:
58761544
Purification:
LSD1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis,Apoptosis
Swissprot #:
O60341
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:

Independent Antibody Validation in Cell lines (Figure 2) shows similar LSD1 expression profile in human and mouse cell lines detected by two independent anti-LSD1 antibodies that recognize different epitopes, 3761 against N-terminus domain and 3763 against central domain. LSD1 proteins are detected in the most tested cell lines that include mouse (3T3/NIH) and rat cell lines (YB2/0) at different expression levels by the two independent antibodies.

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