PAK6 Antibody

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ProSci
Product Code: PSI-3073
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3073-0.02mg0.02mg£150.00
Quantity:
PSI-3073-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of PAK6 in Raji lysate with PAK6 antibody at (A) 1, (B) 2, and (C) 4 μg/mL.
2 / 3
Immunocytochemistry of PAK6 in Raji cells with PAK6 antibody at 10 μg/mL.
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Immunofluorescence of PAK6 in Raji cells with PAK6 antibody at 10 μg/mL.
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Western blot analysis of PAK6 in Raji lysate with PAK6 antibody at (A) 1, (B) 2, and (C) 4 μg/mL.
Immunocytochemistry of PAK6 in Raji cells with PAK6 antibody at 10 μg/mL.
Immunofluorescence of PAK6 in Raji cells with PAK6 antibody at 10 μg/mL.

Documents

Further Information

Additional Names:
PAK6 Antibody: PAK5, PAK5, PAK-5, PAK-6
Application Note:
PAK6 antibody can be used for the detection of PAK6 by Western blot at 1 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
PAK6 Antibody: The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6. These proteins are not activated by Cdc42/Rac binding. PAK6 was initially identified as an androgen receptor in a yeast two hybrid screen and was found to be highly expressed in testis and prostate tissues. Later experiments have shown it to be activated by MAP kinase kinase 6 and p38 MAP kinase, suggesting that PAK6 may play a role in the cellular response to stress-related signals.
Background References:
  • Jaffer ZM and Chernoff J. p21-activated kinases: three more join the Pak. Int. J. Biochem. Cell Biol. 2002; 34:713-7.
  • Yang F, Li X, Sharma M, et al. Androgen receptor specifically interacts with a novel p21-activated kinase, PAK6. J. Biol. Chem. 2001; 276:15345-53.
  • Kaur R, Liu X, Gjoerup O, et al. Activation of p21-activated kinase 6 by MAP kinase kinase 6 and p38 MAP kinase. J. Biol. Chem. 2005; 280:3323-30.
Buffer:
PAK6 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PAK6 antibody was raised against a 13 amino acid synthetic peptide from near the center of human PAK6.

The immunogen is located within amino acids 260 - 310 of PAK6.
NCBI Gene ID #:
56924
NCBI Official Name:
p21 protein (Cdc42/Rac)-activated kinase 6
NCBI Official Symbol:
PAK6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 75 kDa

Observed: 70 kDa
Protein Accession #:
NP_064553
Protein GI Number:
9910476
Purification:
PAK6 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
SPECIFICITY:
PAK6 antibody is predicted to not cross-react with other PAK family proteins.
Swissprot #:
Q9NQU5
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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