PAK5 Antibody
| Code | Size | Price |
|---|
| PSI-3075-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-3075-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
PAK5 Antibody: PAK5, KIAA1264, PAK5, p21-activated kinase 5, PAK-5
Application Note:
PAK5 antibody can be used for the detection of PAK5 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
PAK5 Antibody: The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6. These proteins are not activated by Cdc42/Rac binding. PAK5 was initially identified as a kinase expressed primarily in brain that while possessing a kinase domain and GTPase binding domain similar to PAK4 and PAK6, is completely different from both. Expression of PAK5 in neural based cell lines resulted in neurite outgrowth suggesting that PAK5 may be involved in regulating the cytoskeletal changes necessary for promoting neurite outgrowth. Other experiments suggest that unlike the other PAKs, PAK5 may inhibit apoptosis by phosphorylating the Bcl-2 family member Bad.
Background References:
- Jaffer ZM and Chernoff J. p21-activated kinases: three more join the Pak. Int. J. Biochem. Cell Biol. 2002; 34:713-7.
- Dan C, Nath N, Liberto M, et al. PAK5, a new brain-specific kinase, promotes neurite outgrowth in N1E-115 cells. Mol. Cell. Biol. 2002; 22:567-77.
- Cotteret S, Jaffer ZM, Beeser A, et al. p21-activated kinase 5 (Pak5) localizes to mitochondria and inhibits apoptosis by phosphorylated BAD. Mol. Cell. Biol. 2003; 23:5526-39.
Buffer:
PAK5 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (100%), Rat: (92%)
Immunogen:
PAK5 antibody was raised against a 13 amino acid synthetic peptide from near the center of human PAK5.
The immunogen is located within amino acids 240 - 290 of PAK5.
The immunogen is located within amino acids 240 - 290 of PAK5.
NCBI Gene ID #:
57144
NCBI Official Name:
p21 protein (Cdc42/Rac)-activated kinase 7
NCBI Official Symbol:
PAK7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
Q9P286
Protein GI Number:
12585290
Purification:
PAK5 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction,Homeostasis,Neuroscience
Swissprot #:
Q9P286
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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