Hrk Antibody
| Code | Size | Price |
|---|
| PSI-3771-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-3771-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Documents
Further Information
Additional Names:
Hrk Antibody: DP5, HARAKIRI, BID3, Activator of apoptosis harakiri, BH3-interacting domain-containing protein 3
Application Note:
Hrk antibody can be used for the detection of Hrk by Western blot at 2.5 - 5 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Background:
Hrk Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Hrk, a pro-apoptotic member of the Bcl-2 homology domain-3 (BH3)-only group of the Bcl-2 family of proteins, was also identified as novel protein induced during programmed neuronal death. It lacks significant homology to other Bcl-2 family members except for an 8-amino acid region that is similar to the BH3 motif of Bik. Hrk regulates apoptosis through interaction with the anti-apoptotic proteins Bcl-2 and Bcl-XL via this domain. It does not interact with the pro-apoptotic proteins Bax, Bak, or Bcl-XS. Hrk localizes to mitochondrial membranes in a pattern similar to that previously reported for Bcl-2 and Bcl-XL.
Background References:
- Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
- Imaizumi K, Tsuda M, Imai Y, et al. Molecular cloning of a novel polypeptide, DP5, induced during programmed neuronal death. J. Biol. Chem. 1997; 272:18842-8.
- Inohara N, Ding L, Chen S, et al. harakiri, a novel regulator of cell death, encodes a protein that activates apoptosis and interacts selectively with survival-promoting proteins Bcl-2 and Bcl-XL. EMBO J. 1997; 16:1686-94.
Buffer:
Hrk Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
HRK antibody was raised against a 15 amino acid synthetic peptide from near the center of human Hrk.
The immunogen is located within the last 50 amino acids of Hrk.
The immunogen is located within the last 50 amino acids of Hrk.
NCBI Gene ID #:
8739
NCBI Official Name:
harakiri, BCL2 interacting protein (contains only BH3 domain)
NCBI Official Symbol:
HRK
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 10 kDa
Observed: 12 kDa
Observed: 12 kDa
Protein Accession #:
NP_003797
Protein GI Number:
4504493
Purification:
Hrk Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction,Neuroscience
Swissprot #:
O00198
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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