TNFRSF14 Antibody

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ProSci
Product Code: PSI-3773
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3773-0.02mg0.02mg£150.00
Quantity:
PSI-3773-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of TNFRSF14 in Raji cell lysate with TNFRSF14 antibody at 2 μg/mL in (A) the absence and (B) the presence of blocking peptide.
2 / 4
Immunocytochemistry of TNFRSF14 in Raji with TNFRSF14 antibody at 10 μg/mL.
3 / 4
Immunofluorescence of TNFRSF14 in Raji cells with TNFRSF14 antibody at 20 μg/mL.
4 / 4
Western blot analysis of 5 ng of TNFRSF14 with TNFRSF14 antibody at 1 μg/mL.
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Western blot analysis of TNFRSF14 in Raji cell lysate with TNFRSF14 antibody at 2 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry of TNFRSF14 in Raji with TNFRSF14 antibody at 10 μg/mL.
Immunofluorescence of TNFRSF14 in Raji cells with TNFRSF14 antibody at 20 μg/mL.
Western blot analysis of 5 ng of TNFRSF14 with TNFRSF14 antibody at 1 μg/mL.

Documents

Further Information

Additional Names:
TNFRSF14 Antibody: TR2, ATAR, HVEA, HVEM, CD270, LIGHTR, UNQ329/PRO509, Tumor necrosis factor receptor superfamily member 14, Herpes virus entry mediator A, Herpesvirus entry mediator A
Application Note:
TNFRSF14 antibody can be used for the detection of TNFRSF14 by Western blot at 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
TNFRSF14 Antibody: Tumor necrosis factor receptor (TNFR) superfamily members are defined by cysteine-rich domains in their extracellular regions that bind TNF-related ligands that share a common structural homology in their extracellular domain. TNFRSF14 was initially identified as the Herpesvirus entry mediator and upon binding to the herpes simplex virus (HSV) envelope glycoprotein D or either of its natural ligands LIGHT and lymphotoxin alpha (LT), activates the transcription factors NF-κB and AP-1. Activation of this signal transduction pathway in T cells stimulates T cell proliferation and cytokine production, leading to inflammation and enhanced CTL-mediated tumor immunity, suggesting that these proteins may be useful as potential targets for controlling cellular immune responses.
Background References:
  • Watts TH. TNF/TNFR family members in costimulation of T cell responses. Annu. Rev. Immunol. 2005; 23:23-68.
  • Montgomery RI, Warner MS, Lum BJ, et al. Herpes simplex virus-1 entry into cells mediated by a novel member of the TNF/NGF receptor family. Cell 1996; 87:427-36.
  • Marsters SA, Ayres TM, Skubatch M, et al. Herpesvirus entry mediator, a member of the tumor necrosis factor receptor (TNFR) family, interacts with members of the TNFR-associated factor family and activates the transcription factors NF-κB and AP-1. J. Biol. Chem.1997; 272:14029-32.
  • Mauri DN, Ebner R, Montgomery RI, et al. LIGHT, a new member of the TNF superfamily, and lymphotoxin alpha are ligands for herpesvirus entry mediator. Immunity 1998; 8:21-30.
Buffer:
TNFRSF14 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
TNFRSF14 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human TNFRSF14.

The immunogen is located within the last 50 amino acids of TNFRSF14.
NCBI Gene ID #:
8764
NCBI Official Name:
tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator)
NCBI Official Symbol:
TNFRSF14
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
Q92956
Protein GI Number:
13878821
Purification:
TNFRSF14 Antibody is affinity chromatography purified via peptide column.
Research Area:
Herpes,Chemokines & Cytokines,Infectious Disease
Swissprot #:
Q92956
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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