IL-23 Antibody

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ProSci
Product Code: PSI-3795
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3795-0.02mg0.02mg£150.00
Quantity:
PSI-3795-0.1mg0.1mg£449.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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<strong>Figure 1 Western Blot Validation in Mouse Pancreas Tissue Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: IL-23 3795 (A: 1 μg/mL, B: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 2 Western Blot Validation with Recombinant Protein</strong><br> Loading: 30 ng of human IL-23 recombinant protein per lane. Antibodies: IL-23 3795 (1: 0.25 μg/mL, 2: 0.5 μg/mL, 3: 1 μg/mL and 4: 2μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 3 Western Blot Validation in Mouse Tissue Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: IL-23 3795 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 4 Immunofluorescence Validation of IL-23 in Mouse Pancreas Tissue</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse pancreas tissue labeling IL-23 with 3795 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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<strong>Figure 5 Immunohistochemistry Validation of IL-23 in Mouse Pancreas Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-IL-23 antibody (3795) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚ C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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<strong>Figure 6 Induced Expression Validation of IL-23 in Mouse Keratinocytes of Epidermis (Yoon et al., 2016) </strong><br> The expression of IL-23 protein induced by tape stripping was detected by immunohistochemical analysis of mouse's skin with anti-IL-23 antibodies. Skin sections from the mouse exhibited epidermis staining positive for IL-23 expression 6 hr after tape stripping. Staining with an isotype-matched irrelevant antibody (Ctrl IgG) was used as a negative control.
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<strong>Figure 1 Western Blot Validation in Mouse Pancreas Tissue Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: IL-23 3795 (A: 1 μg/mL, B: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Western Blot Validation with Recombinant Protein</strong><br> Loading: 30 ng of human IL-23 recombinant protein per lane. Antibodies: IL-23 3795 (1: 0.25 μg/mL, 2: 0.5 μg/mL, 3: 1 μg/mL and 4: 2μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation in Mouse Tissue Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: IL-23 3795 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Immunofluorescence Validation of IL-23 in Mouse Pancreas Tissue</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse pancreas tissue labeling IL-23 with 3795 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
<strong>Figure 5 Immunohistochemistry Validation of IL-23 in Mouse Pancreas Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-IL-23 antibody (3795) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚ C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 6 Induced Expression Validation of IL-23 in Mouse Keratinocytes of Epidermis (Yoon et al., 2016) </strong><br> The expression of IL-23 protein induced by tape stripping was detected by immunohistochemical analysis of mouse's skin with anti-IL-23 antibodies. Skin sections from the mouse exhibited epidermis staining positive for IL-23 expression 6 hr after tape stripping. Staining with an isotype-matched irrelevant antibody (Ctrl IgG) was used as a negative control.

Documents

Further Information

Additional Names:
IL-23 Antibody: P19, SGRF, IL-23, IL-23A, IL23P19, UNQ2498/PRO5798, Interleukin-23 subunit alpha, Interleukin-23 subunit p19, IL-23 subunit alpha
Application Note:
WB: 0.25-2 μg/mL; IF: 20 μg/mL, IHC: 2 μg/mL.

Antibody validated: Western Blot in human and mouse samples; Immunofluorescence and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Background:
IL-23 Antibody: Like interleukin-27 (IL-27), IL-23 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the IL-12p40 subunit and a novel p19 subunit. IL-23 is secreted by activated dendritic cells, macrophages, and monocytes. Its biological activities include enhancing the proliferation of memory T cells and the production of IFN-gamma, IL-12, and TNF-α from activated T cells, and can stimulate macrophages to produce TNF-α and nitric oxide. It has also been shown to possess potent anti-tumor and anti-metastatic activity in mouse models of cancer, suggesting a potential role for IL-23 in therapeutic treatment of cancer.
Background References:
  • Hunter. Nat. Rev. Immunol. 2005; 5:521-31.
  • Oppmann et al. Immunity 2000; 13:715-25.
  • Sheibanie et al. FASEB J. 2004; 18:1318-20.
  • Pirhonen et al. J. Immunol. 2002; 169:5673-8.
Buffer:
IL-23 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Horse: (94%), Pig: (82%), Rat: (76%), Guinea pig: (76%)
Immunogen:
Anti-IL-23 antibody (3795) was raised against a peptide corresponding to 17 amino acids near the amino terminus of human IL-23.

The immunogen is located within amino acids 40 - 90 of IL-23.
ISOFORMS:
Human IL-23 has one isoform (189aa, 20.7kD). Mouse IL-23 has one isoform (196aa, 22.1kD) and Rat IL-23 also has one isoform (196aa, 22kD). 3795 can detect human and mouse, and possibly detect rat.
NCBI Gene ID #:
51561
NCBI Official Name:
interleukin 23, alpha subunit p19
NCBI Official Symbol:
IL23A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 21kD

Observed: 22 kD
Protein Accession #:
AAH66268
Protein GI Number:
42542809
Purification:
IL-23 Antibody is affinity chromatography purified via peptide column.
Research Area:
Immunology,Chemokines & Cytokines,Neuroscience
Swissprot #:
Q9NPF7
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:

Recombinant Protein Test (Figure 2): Anti-IL-23 antibodies (3795) detected human IL-23 recombinant protein at different concentrations.

 

Induced Expression Validation (Figure 6): IL-23 expression detected by anit-AIF antibodies was up-regulated by tape stripping.

References

  1. Yoon et al. IL-23 induced in keratinocytes by endogenous TLR4 ligands polarizes dendritic cells to drive IL-22 responses to skin immunization. J Exp Med. 2016;213(10):2147-66. PMID: 27551155

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