TIM-1 Antibody

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ProSci
Product Code: PSI-3811
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3811-0.02mg0.02mg£150.00
Quantity:
PSI-3811-0.1mg0.1mg£449.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)

Images

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<strong>Figure 1 Western Blot Validation in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: TIM-1 3811 (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: TIM-1 3809 (8 μg/mL), TIM-1 3811 (1 μg/mL), beta-actin (1 μg/mL), and GAPDH (0.02 μg/mL), overnight incubation at 4˚ C (3809) or 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 3 Validation with TIM-1 siRNA Knockdown in HeLa Cells</strong><br> HeLa cells were transfected with control siRNAs (lane 1) or TIM-1 siRNAs (lane 2) Loading: 15 μg of HeLa whole cell lysates per lane. Antibodies: 3811 (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 1 Western Blot Validation in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: TIM-1 3811 (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: TIM-1 3809 (8 μg/mL), TIM-1 3811 (1 μg/mL), beta-actin (1 μg/mL), and GAPDH (0.02 μg/mL), overnight incubation at 4˚ C (3809) or 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Validation with TIM-1 siRNA Knockdown in HeLa Cells</strong><br> HeLa cells were transfected with control siRNAs (lane 1) or TIM-1 siRNAs (lane 2) Loading: 15 μg of HeLa whole cell lysates per lane. Antibodies: 3811 (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Documents

Further Information

Additional Names:
TIM-1 Antibody: TIM, KIM1, TIM1, HAVCR, KIM-1, TIM-1, TIMD1, TIMD-1, HAVCR-1, Hepatitis A virus cellular receptor 1, Kidney injury molecule 1, HAVcr-1
Application Note:
WB: 1-8 μg/mL

Antibody validated: Western Blot in human samples. All other applications and species not yet tested
Background:
TIM-1 Antibody: The human form of TIM-1 was initially discovered as a membrane glycoprotein through which the hepatitis A virus can gain entry into a cell. It was also identified as kidney injury molecule 1 (Kim-1), a predicted adhesion molecule that is upregulated on the surfaces of kidney epithelia. It is also expressed on T helper 2 (Th2) cells of the immune system, and following the binding of its natural ligand TIM-4, stimulates T cell expansion and cytokine production in response to viral challenge. It has been suggested that hyperactivation of TIM-1 leads to an increased level of Th2 responsiveness and asthma susceptibility, and antibodies to TIM-1 may therefore be a novel approach to treating asthma.
Background References:
  • Feigelstock et al. J. Virol. 1998; 72:6621-8
  • Ichimura et al. J. Biol. Chem. 1998; 273:4135-42
  • Meyers et al. Mol. Med. 2005; 11:362-9
  • Mayers et al. Nat. Immunol. 2005;6:455-64.
Buffer:
TIM-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (50%), Rat: (43.7%)
Immunogen:
Anti-TIM-1 antibody (3811) was raised against a peptide corresponding to 16 amino acids near the center terminus of human TIM-1.

The immunogen is located within amino acids 230 - 280 of TIM-1.
ISOFORMS:
Human TIM-1 has 5 isoforms, including isoform a precursor (364aa, 39kD), isoform a (364aa, 39kD), isoform b precursor (401aa, 44kD), isoform b (359aa, 39kD), and isoform c (359aa, 39kD). This antibody detects human isoform b precursor. Mouse TIM-1 has 3 isoforms, including isoform a precursor (305aa, 33kD), isoform b precursor (282aa, 31kD) and isoform b (282aa, 31kD). Rat TIM-1 has only one isoform identified so far (307aa, 34kD).
NCBI Gene ID #:
26762
NCBI Official Name:
hepatitis A virus cellular receptor 1
NCBI Official Symbol:
HAVCR1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 44kD

Observed: 60kD (Post-modification: 4 N-linked glycosylation)
Protein Accession #:
NP_036338
Protein GI Number:
153085427
Purification:
TIM-1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Immunology,Neuroscience,Signal Transduction,Growth Factors
Swissprot #:
Q96D42
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:

Independent Antibody Validation in Cell lines (Figure 2) shows similar TIM-1 expression profile in human cell lines detected by two independent anti-TIM-1 antibodies that recognize different epitopes, 3809 against N-terminus domain and 3811 against the central domain. TIM-1 proteins are detected in all the tested cell lines except MCF-7 at different expression levels by the two independent antibodies.  

siRNA knockdown validation (Figure 3): Anti-TIM-1 antibody (3811) specificity was further verified by TIM-1 specific siRNA knockdown. TIM-1 signal in HeLa cells transfected with TIM-1 siRNAs was much weaker in comparison with that in HeLa cells transfected with control siRNAs.

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