Bik Antibody
| Code | Size | Price |
|---|
| PSI-3819-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-3819-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
Bik Antibody: BP4, NBK, BIP1, Bcl-2-interacting killer, Apoptosis inducer NBK
Application Note:
BIK antibody can be used for the detection of BIK by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Bik Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by the activation of proteolytic enzymes termed caspases. Proteins that comprise the Bcl-2 family appear to control the activation of these enzymes. One such protein BIK was recently identified as an endoplasmic reticulum (ER)-residing pro-apoptotic member of the Bcl-2 homology domain-3 (BH3)-only group of the Bcl-2 family that stimulates mitochondrial release of cytochrome c following p53 induction of apoptosis. A significant fraction of BIK is found as an ER transmembrane protein, with most of the protein facing the cytosol. Restricting BIK to the ER membrane by replacing the transmembrane region with that of the ER-selective membrane anchor of cytochrome b resulted in a decreased cytochrome c release from mitochondria and a corresponding drop in cell death. Recent evidence suggests that BIK cooperates with NOXA, another BH3-only protein, to somehow enhance the activation of Bax to stimulate the rapid release of cytochrome c from mitochondria.
Background References:
- Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
- Germain M, Mathai JP, and Shore GC. BH-3-only BIK functions at the endoplasmic reticulum to stimulate cytochrome c release from mitochondria. J. Biol. Chem. 277:18053-60.
- Germain M, Mathai JP, McBride HM, et al. Endoplasmic reticulum BIK initiates DRP1-regulated remodelling of mitochondrial cristae during apoptosis. EMBO J. 2005; 24:1546-56.
Buffer:
Bik Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
BIK antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human BIK.
The immunogen is located within the first 50 amino acids of Bik.
The immunogen is located within the first 50 amino acids of Bik.
NCBI Gene ID #:
638
NCBI Official Name:
BCL2-interacting killer (apoptosis-inducing)
NCBI Official Symbol:
BIK
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 18 kDa
Observed: 22 kDa
Observed: 22 kDa
Protein Accession #:
CAG30276
Protein GI Number:
47678311
Purification:
Bik Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis,Innate Immunity
Swissprot #:
Q13323
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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