TRPC3 Antibody

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ProSci
Product Code: PSI-3895
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3895-0.02mg0.02mg£150.00
Quantity:
PSI-3895-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of TRPC3 in human heart tissue lysate with TRPC3 antibody at (A) 1 and (B) 2 μg/mL.
2 / 2
Immunohistochemistry of TRPC3 in mouse heart tissue with TRPC3 antibody at 10 μg/mL.
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Western blot analysis of TRPC3 in human heart tissue lysate with TRPC3 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of TRPC3 in mouse heart tissue with TRPC3 antibody at 10 μg/mL.

Documents

Further Information

Additional Names:
TRPC3 Antibody: TRP3, TRP3, Short transient receptor potential channel 3, Transient receptor protein 3, TrpC3
Application Note:
TRPC3 antibody can be used for the detection of TRPC3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Background:
TRPC3 Antibody: The mammalian transient receptor potential (TRP) superfamily can be divided into three major families including the "canonical TRP" (TRPC) family. The seven members of this family share the activation through PLC-coupled receptors and have been suggested to be components of receptor-regulated cation channels in different cell types. Furthermore, the members of the TRPC3/6/7 subfamily can be activated by diacylglycerol analogs, suggesting a possible mechanism of activation of these channels by PLC-coupled receptors. TRPC3 encodes a Ca2+-permeant channel that is agonist-activated but not store-operated or directly receptor-activated. TRPC3 physically interacts with TRPC6 and TRPC7 and forms functional tetrameric channels.
Background References:
  • Contell C, Birnbaumer V, Flockerzi V, et al. A unified nomenclature for the superfamily of TRP cation channels. Mol. Cell 2002; 9:229-31.
  • Trebak M, Vazquez G, Bird GSJ, et al. The TRPC3/6/7 subfamily of cation channels. Cell Calcium 2003; 33:451-61.
  • Zitt C, Obukhov AG, Strubing C, et al. Expression of TRPC3 in Chinese hamster ovary cells results in calcium-activated cation currents not related to store depletion. J. Cell. Biol. 1997; 1333-41.
Buffer:
TRPC3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (100%)
Immunogen:
TRPC3 antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human TRPC3.

The immunogen is located within amino acids 120 - 170 of TRPC3.
NCBI Gene ID #:
7222
NCBI Official Name:
transient receptor potential cation channel, subfamily C, member 3
NCBI Official Symbol:
TRPC3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 93 kDa

Observed: 100 kDa
Protein Accession #:
CAA74083
Protein GI Number:
2225937
Purification:
TRPC3 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction,Neuroscience,Infectious Disease
SPECIFICITY:
This antibody may cross-react with TRPC6 and TRPC7.
Swissprot #:
Q13507
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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