TRPC6 Antibody

Product Code: PSI-3897

Supplier: ProSci

Code	Size	Price

PSI-3897-0.02mg

0.02mg

£150.00

Quantity:

PSI-3897-0.1mg

0.1mg

£449.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit

Antibody Isotype: IgG

Antibody Clonality: Polyclonal

Regulatory Status: RUO

Applications:

Enzyme-Linked Immunosorbent Assay (ELISA)
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Western Blot (WB)

Images

1 / 4

Western blot analysis of TRPC6 in mouse lung tissue lysate with TRPC6 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.

2 / 4

Immunohistochemistry of TRPC6 in mouse lung tissue with TRPC6 antibody at 10 μg/mL.

3 / 4

Immunofluorescence of TRPC6 in Mouse Lung cells with TRPC6 antibody at 20 μg/mL.

4 / 4

Immunohistochemistry of TRPC6 in mouse lung tissue with TRPC6 antibody at 5 μg/ml.

❮❯

Documents

Further Information

Additional Names:

TRPC6 Antibody: TRP6, FSGS2, TRP6, Short transient receptor potential channel 6, Transient receptor protein 6, TrpC6

Application Note:

TRPC6 antibody can be used for the detection of TRPC6 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Background:

TRPC6 Antibody: The mammalian transient receptor potential (TRP) superfamily can be divided into three major families including the "canonical TRP" (TRPC) family. The seven members of this family share the activation through PLC-coupled receptors and have been suggested to be components of receptor-regulated cation channels in different cell types. Furthermore, the members of the TRPC6/6/7 subfamily can be activated by diacylglycerol (DAG) analogs, suggesting a possible mechanism of activation of these channels by PLC-coupled receptors. When expressed in transfected cells, TRPC6 acts as a non-selective store-independent receptor-activated cation channel. It is activated by DAG in a PKC-independent manner and is insensitive to IP3 activation. There is increasing evidence that TRPC6 encodes endogenous DAG-activated receptor-operated cation channels in vivo.

Background References:

Contell C, Birnbaumer V, Flockerzi V, et al. A unified nomenclature for the superfamily of TRP cation channels. Mol. Cell 2002; 9:229-31.
Dietrich A, Kalwa H, Rost BR, et al. The diacylglycerol-sensitive TRPC6/6/7 subfamily of cation channels: functional characterization and physiological relevance. Pflugers Arch. 2005; 451:72-80.
Trebak M, Vazquez G, Bird GSJ, et al. The TRPC6/6/7 subfamily of cation channels. Cell Calcium 2003; 33:451-61

Buffer:

TRPC6 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration:

1 mg/mL

Conjugate:

Unconjugated

DISCLAIMER:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Immunogen:

TRPC6 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human TRPC6.

The immunogen is located within the last 50 amino acids of TRPC6.

NCBI Gene ID #:

7225

NCBI Official Name:

transient receptor potential cation channel, subfamily C, member 6

NCBI Official Symbol:

TRPC6

NCBI Organism:

Homo sapiens

Physical State:

Liquid

PREDICTED MOLECULAR WEIGHT:

Predicted: 100, 102 kDa

Observed: 93 kDa

Protein Accession #:

AAH93658

Protein GI Number:

62739910

Purification:

TRPC6 Antibody is affinity chromatography purified via peptide column.

Research Area:

Signal Transduction,Neuroscience

SPECIFICITY:

This antibody may cross-react with TRPC3.

Swissprot #:

Q9Y210

User NOte:

Optimal dilutions for each application to be determined by the researcher.

Related Products

Product Name	Product Code	Supplier
TRPC6 Peptide	PSI-3897P	ProSci	Summary Details