BAG-1 Antibody

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ProSci
Product Code: PSI-3871
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3871-0.02mg0.02mg£150.00
Quantity:
PSI-3871-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of BAG-1 in PC-3 cell lysate with BAG-1 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of BAG-1 in human lymph node tissue with BAG-1 antibody at 2 μg/mL
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Immunofluorescence of BAG-1 in Human Lymph Node cells with BAG-1 antibody at 20 μg/mL.
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Western blot analysis of BAG-1 in PC-3 cell lysate with BAG-1 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of BAG-1 in human lymph node tissue with BAG-1 antibody at 2 μg/mL
Immunofluorescence of BAG-1 in Human Lymph Node cells with BAG-1 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
BAG-1 Antibody: HAP, BAG-1, RAP46, HAP, BAG family molecular chaperone regulator 1, Bcl-2-associated athanogene 1
Application Note:
BAG-1 antibody can be used for the detection of BAG-1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
BAG-1 Antibody: Bcl-2-associated athanogene 1 (BAG-1) was first identified as an anti-apoptotic bcl-2-binding protein. Later it was found to bind the molecular chaperones Hsp70 and Hsc70 through its carboxy-terminal sequence (termed the Bag domain), resulting in the inhibition of the refolding activity of these chaperones. It is thought that by binding and inhibiting these molecular chaperones, BAG-1is able to modulate the expression level of proteins requiring chaperones to fold correctly. One such group of proteins that are affected is glucocorticoid receptors. Other reports have suggested that the level of BAG-1 expression correlates with the aggressiveness of various cancers. Multiple isoforms of BAG-1 are known to exist.
Background References:
  • Takayama S, Sato T, Kraweski K, et al. Cloning and functional analysis of BAG-1: a novel Bcl2-binding protein with anti-cell death activity. Cell 1995; 80:279-84.
  • Nollen EAA, Brunsting JF, Song J, et al. Bag1 functions in vivo as a negative regulator of Hsp70 chaperone activity. Mol. Cell. Biol. 2000; 20:1083-8.
  • Cato ACB and Mink S. BAG-1 family of cochaperones in the modulation of nuclear receptor action. J. Steroid Biochem. & Mol. Biol. 2001; 78:379-88.
  • Kajewska M, Turner BC, Shabaik A, et al. Expression of BAG-1 protein correlates with aggressive behavior of prostate cancers. Prostate 2006; 66:801-10.
Buffer:
BAG-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
BAG-1 antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human BAG-1.

The immunogen is located within the first 50 amino acids of BAG-1.
NCBI Gene ID #:
573
NCBI Official Name:
BCL2-associated athanogene
NCBI Official Symbol:
BAG1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004314
Protein GI Number:
124494251
Purification:
BAG-1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis,Cancer
Swissprot #:
Q99933
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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