TANK Antibody

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ProSci
Product Code: PSI-3879
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3879-0.02mg0.02mg£150.00
Quantity:
PSI-3879-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of TANK in Daudi cell lysate with TANK antibody at (A) 0.5 and (B) 1 μg/mL.
2 / 2
Immunocytochemistry of TANK in Daudi cells with TANK antibody at 2.5 μg/mL.
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Western blot analysis of TANK in Daudi cell lysate with TANK antibody at (A) 0.5 and (B) 1 μg/mL.
Immunocytochemistry of TANK in Daudi cells with TANK antibody at 2.5 μg/mL.

Documents

Further Information

Additional Names:
TANK Antibody: ITRAF, TRAF2, I-TRAF, ITRAF, TRAF family member-associated NF-kappa-B activator, TRAF-interacting protein
Application Note:
TANK antibody can be used for the detection of TANK by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL.

Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Background:
TANK Antibody: TANK was initially identified as a novel TRAF-interacting protein that regulated TRAF-mediated signal transduction. Specifically, ligand binding by surface receptors in the tumor necrosis factor (TNF) receptor and Toll/interleukin-1 (IL-1) receptor families lead to the formation of a TRAF/TANK complex that mediates the activation of the transcription factor NF-κB. This activation of NF-κB occurs through an association with the kinases IKKε and TBK1. More recently, it was shown that these proteins can then form a complex with NEMO, a protein that regulates the activity of the IκB complex. This suggests that in addition to the possibility that TBK1 and IKKε activate the IKKs, the association with the IKK complex may help these kinases modulate other functions, such as the transactivation potential of NF-κB proteins. At least two isoforms of TANK are known to exist.
Background References:
  • Cheng G and Baltimore D. TANK, a co-inducer with TRAF2 of TNF- and CD40L-mediated NF-κB activation. Genes Dev. 1996; 10:963-73.
  • Rothe M, Xiong J, Shu HB, et al. I-TRAF is a novel TRAF-interacting protein that regulates TRAF-mediated signal transduction. Proc. Natl. Acad. Sci. USA 1996; 93:8241-6.
  • Pomerantz JL and Baltimore D. NF-κB activation by a signaling complex containing TRAF2, TANK and TBK1, a novel IKK-related kinase. EMBO J. 1999; 18:6694-704.
  • Chariot A, Leonardi A, Muller J, et al. Association of the adaptor TANK with the IκB kinase (IKK) regulator NEMO connects IKK complexes with the IKKε and TBK1 kinases. J. Biol. Chem.2002; 277:37029-36
Buffer:
TANK Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (79%)
Immunogen:
TANK antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human TANK.

The immunogen is located within amino acids 60 - 110 of TANK.
NCBI Gene ID #:
10010
NCBI Official Name:
TRAF family member-associated NFKB activator
NCBI Official Symbol:
TANK
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004171
Protein GI Number:
19743569
Purification:
TANK Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction,Neuroscience
Swissprot #:
Q92844
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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