Fn14 Antibody

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ProSci
Product Code: PSI-3951
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3951-0.02mg0.02mg£150.00
Quantity:
PSI-3951-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of Fn14 in HepG2 cells with Fn14 antibody at (A) 2 and (B) 4 μg/mL.
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Immunohistochemistry of Fn14 in human liver tissue with Fn14 antibody at 2.5 μg/mL.
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Immunofluorescence of Fn14 in Human Liver cells with Fn14 antibody at 20 μg/mL.
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Western blot analysis of Fn14 in HepG2 cells with Fn14 antibody at (A) 2 and (B) 4 μg/mL.
Immunohistochemistry of Fn14 in human liver tissue with Fn14 antibody at 2.5 μg/mL.
Immunofluorescence of Fn14 in Human Liver cells with Fn14 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
Fn14 Antibody: FN14, CD266, TWEAKR, FN14, Tumor necrosis factor receptor superfamily member 12A, Fibroblast growth factor-inducible immediate-early response protein 14, FGF-inducible 14
Application Note:
Predicted: 62kD

Observed: 68kD
Background:
Fn14 Antibody: TWEAK is a TNF family member produced by IFN-gamma-stimulated monocytes and induces multiple cell death pathways including caspase-dependent apoptosis and cathepsin B-dependent necrosis. These pathways are mediated by the binding of TWEAK by fibroblast growth factor 14 (Fn14), a member of the TNF receptor superfamily. Yeast two-hybrid experiments have shown that FN14 will bind to the TNFR-associated factors TRAF1, TRAF2, TRAF3, and TRAF5, suggesting that the signaling pathway induced by TWEAK may be mediated by one or more of these adaptor molecules. Fn14 is induced during liver regeneration and is highly expressed in hepatocellular carcinomas. In addition, TWEAK induces liver progenitor cell proliferation, suggesting Fn14 may play a role in hepatocyte growth control and liver neoplasia.
Background References:
  • Chicheportiche Y, Bourdon PR, Xu H, et al. TWEAK, a new secreted ligand in the tumor necrosis factor family that weakly induces apoptosis. J. Biol. Chem.1997; 272:32401-10.
  • Nakayama M, Ishidoh K, Kojima Y, et al. Fibroblast growth factor-inducible 14 mediates multiple pathways of TWEAK-induced cell death. J. Immunol. 2003; 170:341-8.
  • Brown SA, Richards CM, Hanscom HN, et al. The Fn14 cytoplasmic tail binds tumour-necrosis-factor-receptor-associated factors 1, 2, 3, and 5 and mediates nuclear factor-kappaB activation. Biochem. J. 2003; 371:395-403.
  • Feng SL, Guo Y, Factor VM, et al. The Fn14 immediate-early response gene is induced during liver regeneration and highly expressed in both human and murine hepatocellular carcinomas. Am. J. Pathol. 2000; 156:1253-61
Buffer:
Fn14 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Fn14 antibody was raised against a human IgG1 Fc-tagged Fn14 protein.
NCBI Gene ID #:
51330
NCBI Official Name:
tumor necrosis factor receptor superfamily, member 12A
NCBI Official Symbol:
TNFRSF12A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_057723
Protein GI Number:
7706186
Purification:
Fn14 Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis
Swissprot #:
Q9NP84
User NOte:
Optimal dilutions for each application to be determined by the researcher.