RIG-1 Antibody
| Code | Size | Price |
|---|
| PSI-3953-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-3953-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
RIG-1 Antibody: RIGI, RIG-I, RLR-1, Probable ATP-dependent RNA helicase DDX58, DEAD box protein 58
Application Note:
RIG-1 antibody can be used for the detection of RIG-1 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human and rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human and rat samples. All other applications and species not yet tested.
Background:
RIG-1 Antibody: The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the cytoplasmic helicase retinoic acid inducible gene protein 1 (RIG-1) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. Unlike TLR3 however, RIG-1 activates the kinases TBK1 and IKKε through the adaptor protein IPS-1. These kinases then phosphorylate the transcription factors IRF-3 and IRF-7 which are essential for the expression of type-I interferons. RIG-1 is required for the production of interferons in response to RNA viruses including paramyxoviruses, influenza virus, and Japanese encephalitis virus.
Background References:
- Akira S, Uematsu S, and Takeuchi O. Pathogen recognition and innate immunity. Cell 2006; 124:783-801.
- Yoneyama M, Kikuchi M, Natsukawa T, et al. The RNA helicase RIG-I has an essential function in double-stranded RNA-induced innate antiviral responses. Nat. Immunol. 2004; 5:730-7.
- Alexopoulou L, Holt AC, Medzhitov R, et al. Recognition of double-stranded RNA and activation of NF-kappaB by Toll-like receptor 3. Nature 2001; 413:732-8.
- Sharma S, tenOever BR, Grandvaux N, et al. Triggering the interferon antiviral response through an IKK-related pathway. Science 2003; 300:1148-51.
Buffer:
RIG-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
RIG-1 antibody was raised against human GST-tagged RIG-1 protein.
NCBI Gene ID #:
23586
NCBI Official Name:
DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
NCBI Official Symbol:
DDX58
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
O95786
Protein GI Number:
81170421
Purification:
RIG-1 Antibody is Protein A purified.
Research Area:
Infectious Disease
Swissprot #:
O95786
User NOte:
Optimal dilutions for each application to be determined by the researcher.